Large-scale egg yolk antibody production method capable of reducing caprylic acid egg residue residues

A technology of egg yolk antibody and caprylic acid, which is applied in the preparation methods of peptides, antibodies, chemical instruments and methods, etc., can solve the problems of strong irritation, animals do not eat, and sudden gastrointestinal diseases, etc. The effect of large dissolved amount, reducing the pressure of resource waste and environmental pollution

Pending Publication Date: 2022-06-21
GUANGDONG HAID ANIMAL HUSBANDRY & VETERINARY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the strong taste of pungent acid and the strong irritation to the gastrointestinal tract, after adding it to the feed and feeding it to animals, there are often phenomena such as animals not eating or sudden gastrointestinal diseases, so it is difficult to add pungent acid egg slag as feed. feed mode reuse

Method used

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  • Large-scale egg yolk antibody production method capable of reducing caprylic acid egg residue residues
  • Large-scale egg yolk antibody production method capable of reducing caprylic acid egg residue residues

Examples

Experimental program
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Effect test

Embodiment 1

[0041] A method that can be used for large-scale production of goose plague yolk antibody, comprising the following steps:

[0042] (1) 1000 gosling plague-free eggs (purchased from Shandong Ennoji Bioengineering Co., Ltd.) were soaked and disinfected with 0.1% Xinjieermin for 15 minutes;

[0043] (2) mechanically beating the cleaned and sterilized high-free eggs to obtain egg yolks;

[0044] (3) inject the egg yolk obtained in the step (2) into the egg stirring tank, the egg yolk is stirred and mixed, and the stirring speed is 200rpm to obtain egg yolk liquid;

[0045] (4) Under the condition of 25°C, prepare the glacial acetic acid-sodium acetate buffer solution of 4.4 times the volume of egg yolk liquid in the acidification tank, and adjust the pH to 5.4 with sodium hydroxide;

[0046] (5) the prepared acidified water is injected into the egg yolk liquid prepared in step (3), stirring time is 30min, stirring speed is 130rpm, after stirring, leave standstill at room tempera...

Embodiment 2

[0052] A method that can be used for large-scale production of goose plague yolk antibody, comprising the following steps:

[0053] (1) 1000 gosling plague-free eggs (purchased from Shandong Ennoji Bioengineering Co., Ltd.) were soaked and disinfected with 0.1% Xinjieermin for 15 minutes;

[0054] (2) mechanically beating the cleaned and sterilized high-free eggs to obtain egg yolks;

[0055] (3) inject the egg yolk obtained in the step (2) into the egg stirring tank, the egg yolk is stirred and mixed, and the stirring speed is 200rpm to obtain egg yolk liquid;

[0056] (4) Under the condition of 25°C, prepare 5.2 times the volume of glacial acetic acid-sodium acetate buffer solution of egg yolk in an acidifying tank, and adjust the pH to 5.0 with sodium hydroxide;

[0057] (5) the prepared acidified water is injected into the egg yolk liquid prepared in step (3), stirring time is 30min, stirring speed is 160rpm, after stirring, leave standstill at room temperature for overni...

Embodiment 3

[0063] A method that can be used for large-scale production of goose plague yolk antibody, comprising the following steps:

[0064] (1) 1000 gosling plague-free eggs (purchased from Shandong Ennoji Bioengineering Co., Ltd.) were soaked and disinfected with 0.1% Xinjieermin for 15 minutes;

[0065] (2) mechanically beating the cleaned and sterilized high-free eggs to obtain egg yolks;

[0066] (3) inject the egg yolk obtained in the step (2) into the egg stirring tank, the egg yolk is stirred and mixed, and the stirring speed is 300rpm to obtain egg yolk liquid;

[0067] (4) Under the condition of 25°C, prepare 5.8 times the volume of glacial acetic acid-sodium acetate buffer solution of egg yolk in an acidifying tank, and adjust the pH to 4.6 with sodium hydroxide;

[0068] (5) the prepared acidified water is injected into the egg yolk liquid prepared in step (3), stirring time is 25min, stirring speed is 100rpm, after stirring, leave standstill at room temperature for overni...

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Abstract

The invention belongs to the technical field of biological products, discloses a large-scale egg yolk antibody production method capable of reducing caprylic acid egg residue residues, and particularly discloses an egg yolk antibody preparation method which comprises the following steps: mixing an egg yolk liquid with an acetic acid-acetate buffer solution, and carrying out solid-liquid separation to obtain an egg yolk antibody liquid; mixing the yolk antibody solution with caprylic acid, extracting, and collecting lower-layer clear liquid; and mixing the lower-layer clarified liquid with formaldehyde to obtain the egg yolk antibody. According to the preparation method of the egg yolk antibody provided by the invention, egg yolk antibody extraction operation is divided into two steps of acidified water precipitation extraction and octanoic acid precipitation extraction, firstly, acetic acid-acetate is used as a main ionic medium, so that the yield of the egg yolk antibody is increased, and available egg residues are obtained; the octanoic acid extraction step is used as an operation means for further removing lipoprotein in egg yolk, the waste amount of octanoic acid egg residues can be reduced by about 65%-70%, and the output of the octanoic acid egg residues is reduced to a great extent.

Description

technical field [0001] The invention belongs to the technical field of biological products, and in particular relates to a method for large-scale production of egg yolk antibody which can reduce the residue of caprylic acid egg residue. Background technique [0002] Bacterial and viral diseases of livestock, poultry and aquatic products, and bacterial diseases caused by the late development of viral diseases hinder the healthy development of the breeding industry. Although the use of antibiotics has greatly controlled bacterial diseases in livestock and aquatic products, and bacterial secondary infections caused by viral diseases in the later stage, the abuse of antibiotics has caused environmental pollution and seriously endangered human health. Since the implementation of the ban on growth-promoting antibiotics in feed in July 2020, a comprehensive ban on antibiotics has officially kicked off. Egg yolk antibody has become one of the effective substitutes for antibiotics b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/10C07K16/08C07K16/02C07K1/36C07K1/34C07K1/30A23K20/147A61K39/42A61P31/14A61P1/16A61P31/20
CPCC07K16/1009C07K16/10C07K16/081C07K16/02A23K20/147A61P31/14A61P1/16A61P31/20C07K2317/11
Inventor 刘田李硕袁建丰
Owner GUANGDONG HAID ANIMAL HUSBANDRY & VETERINARY RES INST
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