Chitosan enhanced enzyme induced carbonate precipitation curing agent and application method thereof
A technology of carbonate precipitation and chitosan, applied in chemical instruments and methods, biochemical equipment and methods, fixed/solidified sludge treatment, etc., to achieve the effect of less repair times, high repair efficiency, and guaranteed repair efficiency
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[0039] In the present invention, the activity of urease in the urease solution is 342.7U / g.
[0040] Among the present invention, the preparation method of described chitosan colloidal solution comprises the following steps:
[0041] Step 1, under the condition of magnetic stirring, chitosan is added in the acetic acid solution, continuously stirred until there is no particle, and the glue is obtained; the degree of deacetylation of the chitosan is 75%~95%; the volume of the acetic acid solution is 50 to 400 times the mass of chitosan, the volume unit of the acetic acid solution is mL, the unit of the chitosan mass is g, and the mass percent concentration of the acetic acid solution is 1% to 10%;
[0042]Step 2. Under magnetic stirring conditions, add sodium hydroxide solution into the glue solution described in step 1 until the pH is 5.5 to 6.8, and continue to stir until there are no particles and the system is clear and transparent to obtain a chitosan colloid solution; the...
Embodiment 1
[0056] Example 1
[0057] The present embodiment provides a chitosan-enhanced enzyme-induced carbonate precipitation curing agent, comprising urease solution, chitosan colloidal solution and urea solution; the volume of the chitosan colloidal solution is 9 times the volume of the urease solution, so The volume of the urea solution is 5 times the volume of the urease solution; the concentration of the urease solution is 60g / L; the concentration of the urea solution is 2mol / L.
[0058] In this example, the activity of urease in the urease solution is 342.7 U / g.
[0059] In the present embodiment, the preparation method of described chitosan colloidal solution comprises the following steps:
[0060] Step 1, under the condition of magnetic stirring, add 0.9g chitosan into 90mL acetic acid solution, continue to stir until there are no particles, and obtain a glue; the degree of deacetylation of the chitosan is 75% to 95%; the acetic acid solution The mass percentage concentration...
Embodiment 2
[0078] Example 2
[0079] The present embodiment provides a chitosan-enhanced enzyme-induced carbonate precipitation curing agent, comprising urease solution, chitosan colloidal solution and urea solution; the volume of the chitosan colloidal solution is 9 times the volume of the urease solution, so The volume of the urea solution is 5 times the volume of the urease solution; the concentration of the urease solution is 60g / L; the concentration of the urea solution is 2mol / L.
[0080] In this example, the activity of urease in the urease solution is 342.7 U / g.
[0081] In the present embodiment, the preparation method of described chitosan colloidal solution comprises the following steps:
[0082] Step 1, under the condition of magnetic stirring, add 0.9g chitosan into 90mL acetic acid solution, continue to stir until there are no particles, and obtain a glue; the degree of deacetylation of the chitosan is 75% to 95%; the acetic acid solution The mass percentage concentration...
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