Method for separating compound by off-line three-dimensional high-speed counter-current chromatography

Abstract

The invention provides a method for off-line three-dimensional high-speed counter-current chromatography separation of compounds. The invention also provides a method for separating the neolignan compound from the betel. According to the off-line three-dimensional high-speed counter-current chromatography compound separation method provided by the invention, the separation effect of a previous solvent system is kept by an in-situ concentration technology, and rapid separation of compounds with similar structures is realized by combining online storage and multi-stage circulating elution.

Description

technical field [0001] The invention relates to the technical field of natural medicines, in particular to a method for separating compounds by off-line three-dimensional high-speed countercurrent chromatography. More specifically, the present invention relates to a method for the separation and purification of compounds of natural origin using off-line three-dimensional high-speed countercurrent chromatography. Background technique [0002] High-speed countercurrent chromatography (HSCCC) was first proposed by Ito et al. in the early 1960s, and then gradually developed. It is a unique solid support-free, liquid-liquid partition chromatography with the ability to eliminate irreversible samples on solid supports. The characteristic of adsorption is that the stationary phase is the mobile phase, which is a commonly used recyclable organic reagent. Compared with traditional separation techniques, HSCCC has the advantages of fast and efficient separation, simple operation, low ...

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Problems solved by technology

The prior art records that neolignans have synergistic anti-drug-resistant Staphylococcus aureus activity, but due to the structural similarity of these compounds, conventional separation methods are difficult to separate and the sample loss is large (J.Org.Chem.2021,86,16 , 11072-11085 Publication Date: January 13, 2021, https: / / doi.org / 10.1021 / acs.joc.0c02682)

[0004] The study found that there is no report on the use of HSCCC to separate the effective chemical components of betel leaves

In addition, macroporous resin, silica gel, sephadex LH-20 and preparative HPLC are commonly used to separate betel leaves, but the separation method is cumbersome and consumes a lot of solvent, which means high risk of irreversible sample adsorption and low yield of sample recovery

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