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Application of Alistipes putredinis bacteria in preparation of medicine for preventing and treating inflammatory bowel disease

A technology for inflammatory bowel disease and drugs, applied in the field of preparation of drugs for the prevention and treatment of inflammatory bowel disease, to achieve the effect of reducing disease activity index, reducing intestinal mucosal damage and inflammation, and reducing symptoms

Pending Publication Date: 2022-06-03
THE THIRD XIANGYA HOSPITAL OF CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Intestinal flora is an important link in the mutual regulation of host and environmental factors, and is closely related to the pathogenesis of CD. However, there are few records on the use of intestinal flora to treat CD-related diseases

Method used

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  • Application of Alistipes putredinis bacteria in preparation of medicine for preventing and treating inflammatory bowel disease
  • Application of Alistipes putredinis bacteria in preparation of medicine for preventing and treating inflammatory bowel disease
  • Application of Alistipes putredinis bacteria in preparation of medicine for preventing and treating inflammatory bowel disease

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preparation example Construction

[0020] The inflammatory bowel disease of the present invention preferably includes Crohn's disease. The active ingredient of the medicament of the present invention preferably includes the supplemented Alistipes putredinis bacterial liquid. The preparation method of the supplemented Alistipes putredinis bacterial liquid of the present invention preferably includes: taking the Alistipes putredinis bacterial liquid activated and cultured in a medium for 3 days, collecting the bacterial cells after centrifugation, and resuspending with anaerobic PBS to an OD value of 1.

[0021] The preparation method of the Alistipes putredinis bacterial liquid of the present invention preferably includes inoculating the dry powder of Alistipes putredinis into a medium for 3d activation culture, and the medium is based on a BHI (brain-heartinfusion broth, brain-heart infusion) medium. The medium also includes 5 μg / mL heme and 0.5 μg / mL vitamin K1. The preparation method of the culture medium of...

Embodiment 1

[0028] A. putredinis abundance is significantly decreased in the gut of CD patients

[0029] Metagenomics analysis of stool samples from 30 pre-treatment CD patients and 30 healthy controls was entrusted to Zhongke New Life.

[0030] The result is as figure 1 shown, the abundance of A. putredinis was significantly reduced in CD patients compared with healthy controls ( figure 1 in A-B). At the same time, the Gram stain of A. putredinis was negative ( figure 1 middle C). Scanning electron microscope observation showed that A. putredinis was a typical rod-shaped bacteria ( figure 1 middle D).

Embodiment 2

[0032] A. putredinis inhibits intestinal inflammation in DSS colitis mice

[0033] like figure 2 The experimental design shown in middle A: C57BL / 6 mouse DSS colitis model (Wirtz S, Popp V, Kindermann M, Gerlach K, Weigmann B, Fichtner-Feigl S, Neurath MF. Chemically induced mouse models of acute and chronic intestinal intestinal was used) inflammation.NatProtoc.2017Jul;12(7):1295-1309), A.putredinis was administered by gavage (take 1×10 9 CFU / mL bacterial solution was centrifuged at 4000 rpm for 10 min at 4°C to collect the bacterial pellet, then resuspended with anaerobic PBS to an OD value of 1, and administered 200 μL / gavage).

[0034] The results are shown in Tables 1 to 5 and figure 2As shown, DSS colitis mice showed different degrees of weight loss, and A. putredinis inhibited the weight loss, and the weight loss rate from the 4th day was lower than that of DSS colitis mice ( figure 2 in B-C). In addition, A. putredinis significantly down-regulated the symptoms o...

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Abstract

The invention provides application of Alistipes putredinis bacteria in preparation of a medicine for preventing and treating inflammatory bowel diseases, and relates to the technical field of biological medicines. A C57BL / 6 mouse colitis model is adopted, and it is found that the A.putredinis bacterium can improve intestinal inflammation of DSS mice. It is confirmed for the first time that A.putredinis can inhibit intestinal inflammation of mice with DSS colitis, a new theory is provided for pathogenesis of CD, a new treatment strategy is provided for CD diagnosis and treatment, and potential clinical application prospects are achieved.

Description

Technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to the application of Alistipes putredinis bacteria in the preparation of drugs for preventing and treating inflammatory bowel disease. Background technique [0002] Crohn's disease (CD) is a chronic relapsing intestinal inflammatory disease. The incidence of CD has increased significantly in my country in recent years. The affected population is younger and the curative effect is poor. Patients with CD often develop intestinal obstruction, fistulas and abdominal cavity. Complications such as abscess require surgical treatment and the prognosis is poor. The pathogenesis of CD is complex and unclear. Revealing the pathogenesis of CD is of great significance for finding new targets and new strategies for CD treatment. [0003] Intestinal flora is an important link in the mutual regulation of host and environmental factors and is closely related to the onset of CD. Howev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/741A61P1/00A61P29/00
CPCA61K35/741A61P1/00A61P29/00Y02A50/30
Inventor 王晓艳邓敏子罗薇薇聂凯吴兴徐嘉豪陈学杰张超马可嘉
Owner THE THIRD XIANGYA HOSPITAL OF CENT SOUTH UNIV
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