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Production of lactase using regulatory altered strains

A technology of lactase and lactose, applied in the direction of glycosylase, using vectors to introduce foreign genetic material, enzymes, etc.

Pending Publication Date: 2022-05-17
CHR HANSEN AS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Furthermore, known lactases are not suitable for use in the process required to produce ultra-high temperature treated (UHT) milk, where the enzyme is added prior to UHT treatment

Method used

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  • Production of lactase using regulatory altered strains
  • Production of lactase using regulatory altered strains
  • Production of lactase using regulatory altered strains

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Embodiment approach

[0039] The present invention relates to a method for producing an enzyme having lactase activity, wherein the enzyme is produced in a lactic acid bacterium, preferably, wherein the bacterium carries a mutation in the DNA sequence of the glcK gene encoding the glucokinase protein, wherein the mutation makes the Glucokinase protein inactivation, the method comprises the following steps:

[0040] a) inoculating the lactic acid bacteria in a suitable growth medium, preferably a suitable growth medium comprising lactose;

[0041] b) growing the lactic acid bacteria of step a) to a desired density to obtain a first solution comprising the enzyme with lactase activity and bacterial cells from the lactic acid bacteria;

[0042] c) inactivating and / or destroying the bacterial cells of the lactic acid bacteria to obtain a second solution;

[0043] d) optionally treating the second solution to obtain a third solution.

[0044] The present invention also relates to a method for producin...

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Abstract

The present invention relates to a novel improved method of expressing these natural lactases in a natural host of the natural lactases. The invention comprises a method for homologous and heterologous expression of lactase in lactic acid bacteria with altered expression kinetics.

Description

technical field [0001] The present invention relates to novel and improved methods for expressing natural lactases in their natural hosts. Here we describe methods for the homologous and heterologous expression of lactase in lactic acid bacteria with altered expression kinetics. Background technique [0002] For growth on milk, lactose hydrolysis is a good way for lactic acid bacteria to obtain glucose and galactose as carbon sources. Lactase (β-galactosidase; EC 3.2.1.23) is an enzyme that performs the hydrolysis step of hydrolyzing the sugar lactose in milk into monosaccharides. Lactase is used commercially to break down lactose in dairy products. People who are lactose intolerant have difficulty digesting dairy products with high lactose levels. It is estimated that about 70% of the world's population has a limited ability to digest lactose. Therefore, there is a growing demand for dairy foods that are lactose-free or contain only low levels of lactose. [0003] Lact...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/24C12N15/74C12N15/54C12N15/56A23C9/12A23C13/16A23C15/12A23C19/06
CPCC12N9/2402C12N15/746C12N9/1205C12N9/2471C12Y302/01108C12Y207/01002C12Y302/01023A23C9/1206A23C13/16A23C15/123A23C19/063
Inventor H·拉杰C·E·G·苏勒K·I·瑟伦森约翰尼斯·马腾·范丹尼布林克A·扎伊丹M·H·劳P·加斯帕G·N·伯罗伊
Owner CHR HANSEN AS
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