Umami peptide, composition containing umami peptide and application of umami peptide
A technology of umami peptides and uses, applied in the field of salt substitutes, can solve problems such as increasing the risk of heart disease, affecting food flavor, high blood pressure, etc. Effect
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Synthetic example 1
[0041] Entrusted Nanjing Yuanpeptide Biotechnology Co., Ltd. to synthesize the umami peptide TPLVDR.
[0042] The purity of the synthesized TPLVDR was detected by HPLC (equipment model is Agilent 1100), such as figure 1 ( figure 1 As shown in the HPLC chromatogram of umami peptide TPLVDR), the purity is 98.72%.
[0043] Use HPLC-MS (equipment model AB SCIEX X500R Q-TOF) to determine the molecular weight and structure, such as figure 2 ( figure 2 It is the secondary mass spectrometry analysis diagram of the umami peptide TPLVDR), showing that the molecular weight is 699.39 Da, and the structure is H-Thr-Pro-Leu-Val-Asp-Arg-OH.
Synthetic example 2
[0045] Entrusted Nanjing Yuanpeptide Biotechnology Co., Ltd. to synthesize the freshness-enhancing peptide AEINKILGN.
[0046] The purity of the synthesized AEINKILGN was detected by HPLC (equipment model is Agilent 1100), as image 3 ( image 3 As shown in the HPLC chromatogram of the freshening peptide AEINKILGN), the purity is 98.96%.
[0047]Use HPLC-MS / MS (equipment model AB SCIEX X500R Q-TOF) to determine the molecular weight and structure, such as Figure 4 ( Figure 4 It is the secondary mass spectrometry analysis diagram of the freshness-enhancing peptide AEINKILGN) showing that the molecular weight is 970.54Da and the structure is H-Ala-Glu-Ile-Asn-Lys-Ile-Leu-Gly-Asn-OH.
[0048] Taste analysis:
[0049] 14 healthy sensory evaluation team members (8 females and 6 males, aged 24-30 years) with experience in sensory evaluation were recruited from the laboratory. First, a 2-week taste training was conducted for the members of the evaluation team. The training cont...
Embodiment 1
[0054] A salt substitute mixture, including 1mol umami-taste peptide TPLVDR and 9mol umami-enhancing peptide AEINKILGN.
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