Microalgae nutrition repair liquid for relieving grassland desertification
A technology of remediation fluid and microalgae, applied in microorganisms, unicellular algae, microorganism-based methods, etc., can solve the problems of reducing the number and activity of soil microorganisms, inhibiting the development of plant roots, affecting plant growth, etc., to prevent plant diseases and insect pests, The effect of improving plant stress resistance and high cultivation efficiency
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Embodiment 1
[0029] A method for cultivating a microalgae nutrient repair solution for relieving grassland desertification, comprising the following steps:
[0030] S1, in a sterile environment, the light intensity is 2300 lux, and the temperature is 25 ℃, using purified water, respectively, expand the cultivation of Thar desert silk algae, Coleoptera Monofidium, nitrogen-fixing Anabaena and Chlorella pyrenoidosa, seven kinds of algal liquid were obtained, among which,
[0031] The expansion and cultivation process of the seven kinds of algal fluids all included: selection and isolation of single cells—the single cells were cultured in a 15 mL test tube for 10 days, and the number of active cells of the microalgae was 10 6 1 / mL—transfer to a 100mL flask for culturing for 10 days, and the number of microalgae active cells is 10 6 cells / mL—transfer to a 1L flask for 10 days, and the number of microalgae viable cells is 10 6 Cells / mL—inoculated into a 5L flask for 10 days, and the number of...
Embodiment 2
[0035] A method for cultivating a microalgae nutrient repair solution for relieving grassland desertification, comprising the following steps:
[0036] S1, in a sterile environment, the light intensity is 25001ux, and the temperature is 26 ℃, using purified water to expand the cultivation of Thar desert silk algae, T. Monofidium, nitrogen-fixing Anabaena and Chlorella pyrenoidosa, seven kinds of algal liquid were obtained, among which,
[0037] The expansion and cultivation process of the seven kinds of algal fluids all included: selection and isolation of single cells - single cells were cultured in a 15 mL test tube for 12 days, and the number of microalgal active cells was 106 Cells / mL—inoculated into 100mL flasks for 12 days, and the number of microalgae viable cells was 10 6 cells / mL—transfer to a 1L flask for 12 days, and the number of microalgae viable cells is 10 6 cells / mL—transfer to a 5L flask and cultivate for 12 days, the number of microalgae active cells is 10 ...
Embodiment 3
[0041] A method for cultivating a microalgae nutrient repair solution for relieving grassland desertification, comprising the following steps:
[0042] S1, in a sterile environment, the light intensity is 27001ux, and the temperature is 27 °C, using purified water, respectively, expand the cultivation of Thar desert silk algae, T. Monofidium, nitrogen-fixing Anabaena and Chlorella pyrenoidosa, seven kinds of algal liquid were obtained, among which,
[0043] The expansion and cultivation process of the seven kinds of algal fluids all included: selection and isolation of single cells - single cells were cultured in a 15 mL test tube for 15 days, and the number of microalgal active cells was 10 6 cells / mL—transfer to a 100mL flask for 15 days, and the number of microalgae viable cells is 10 6 cells / mL—transfer to a 1L flask for 15 days, and the number of microalgae viable cells is 10 6 Cells / mL—inoculated into a 5L flask for 15 days, and the number of microalgae viable cells wa...
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