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Microalgae nutrition repair liquid for relieving grassland desertification

A technology of remediation fluid and microalgae, applied in microorganisms, unicellular algae, microorganism-based methods, etc., can solve the problems of reducing the number and activity of soil microorganisms, inhibiting the development of plant roots, affecting plant growth, etc., to prevent plant diseases and insect pests, The effect of improving plant stress resistance and high cultivation efficiency

Active Publication Date: 2022-05-13
内蒙古阿尔格生命科学有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the soil in desertified areas is poor, and the nutrients that can provide vegetation are seriously lacking, the survival rate of vegetation is low, and the control effect is not good.
The application of chemical fertilizers can improve the survival rate of vegetation to a certain extent, but the negative impact of chemical fertilizers cannot be ignored: the raw materials for the production of chemical fertilizers contain heavy metals such as Zn, Cu, Co, and Cr, and these heavy metals are added to the plant following chemical fertilizers. soil, and cause the enrichment of heavy metal elements in the soil, which enter the plant through plant absorption and affect plant growth; chemical fertilizers will reduce the number and activity of soil microorganisms, thereby reducing the conversion of soil microorganisms to organic matter in the soil, the decomposition of minerals and Degradation of toxic substances; long-term application of chemical fertilizers will aggravate soil acidification, aggravate soil compaction, inhibit plant root development, and seriously reduce plant stress resistance

Method used

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  • Microalgae nutrition repair liquid for relieving grassland desertification
  • Microalgae nutrition repair liquid for relieving grassland desertification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for cultivating a microalgae nutrient repair solution for relieving grassland desertification, comprising the following steps:

[0030] S1, in a sterile environment, the light intensity is 2300 lux, and the temperature is 25 ℃, using purified water, respectively, expand the cultivation of Thar desert silk algae, Coleoptera Monofidium, nitrogen-fixing Anabaena and Chlorella pyrenoidosa, seven kinds of algal liquid were obtained, among which,

[0031] The expansion and cultivation process of the seven kinds of algal fluids all included: selection and isolation of single cells—the single cells were cultured in a 15 mL test tube for 10 days, and the number of active cells of the microalgae was 10 6 1 / mL—transfer to a 100mL flask for culturing for 10 days, and the number of microalgae active cells is 10 6 cells / mL—transfer to a 1L flask for 10 days, and the number of microalgae viable cells is 10 6 Cells / mL—inoculated into a 5L flask for 10 days, and the number of...

Embodiment 2

[0035] A method for cultivating a microalgae nutrient repair solution for relieving grassland desertification, comprising the following steps:

[0036] S1, in a sterile environment, the light intensity is 25001ux, and the temperature is 26 ℃, using purified water to expand the cultivation of Thar desert silk algae, T. Monofidium, nitrogen-fixing Anabaena and Chlorella pyrenoidosa, seven kinds of algal liquid were obtained, among which,

[0037] The expansion and cultivation process of the seven kinds of algal fluids all included: selection and isolation of single cells - single cells were cultured in a 15 mL test tube for 12 days, and the number of microalgal active cells was 106 Cells / mL—inoculated into 100mL flasks for 12 days, and the number of microalgae viable cells was 10 6 cells / mL—transfer to a 1L flask for 12 days, and the number of microalgae viable cells is 10 6 cells / mL—transfer to a 5L flask and cultivate for 12 days, the number of microalgae active cells is 10 ...

Embodiment 3

[0041] A method for cultivating a microalgae nutrient repair solution for relieving grassland desertification, comprising the following steps:

[0042] S1, in a sterile environment, the light intensity is 27001ux, and the temperature is 27 °C, using purified water, respectively, expand the cultivation of Thar desert silk algae, T. Monofidium, nitrogen-fixing Anabaena and Chlorella pyrenoidosa, seven kinds of algal liquid were obtained, among which,

[0043] The expansion and cultivation process of the seven kinds of algal fluids all included: selection and isolation of single cells - single cells were cultured in a 15 mL test tube for 15 days, and the number of microalgal active cells was 10 6 cells / mL—transfer to a 100mL flask for 15 days, and the number of microalgae viable cells is 10 6 cells / mL—transfer to a 1L flask for 15 days, and the number of microalgae viable cells is 10 6 Cells / mL—inoculated into a 5L flask for 15 days, and the number of microalgae viable cells wa...

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Abstract

The invention discloses a microalgae nutrition repair liquid for relieving grassland desertification, which comprises a microalgae mixed liquid, the microalgae mixed liquid comprises seven active microalgae, and the added microalgae comprises the following components in parts by weight: 5-40 parts of hylothrix tadeeri, 5-40 parts of hylothrix wonderi, 10-50 parts of microcoleus vaginalis, 5-40 parts of pseudocladoidea, 10-50 parts of monofidus microfidus and 30-80 parts of chlorella pyrenoidosa, and the microalgae mixed liquid comprises the following components in parts by weight: 5-40 parts of hylothrix tadeeri, 5-40 parts of hylothrix wonderi, 10-50 parts of microcoleus vaginalis and 30-80 parts of chlorella pyrenoidosa. In addition, 10-50 parts of nitrogen-fixing anabaena or 20 parts of cotton algae are selected to be added. Aiming at the grassland desertification region, the nutrient restoration liquid can restore soil, supplement natural nitrogen, activate soil trace elements, improve plant stress resistance and prevent plant diseases and insect pests, the seven microalgae have good compatibility, the number of microalgae active cells in each 1mL of the nutrient restoration liquid can reach more than 106, and the nutrient restoration liquid has good application prospects. The storage life of the nutrient repair liquid reaches 18 months, the culture process is obtained through a large number of experiments, and the culture efficiency is high.

Description

technical field [0001] The invention belongs to the technical field of desertification prevention and control, in particular to a microalgae nutrient repair solution for relieving grassland desertification. Background technique [0002] At present, two-thirds of the world's countries and regions face the threat of desertification, which seriously affects the living space and sustainable development of human beings. Desertification has become an ecological and environmental issue of global concern. In desertified areas, land is degraded, productivity declines, surface wind erosion and sandification, sand dunes move and bury farmland, roads, and houses, the ecology is fragile, the economic conditions are relatively backward, and prevention and control are very difficult. [0003] Improving the vegetation environment is an important means to prevent desertification, such as afforestation, grass breeding and grass cultivation. However, the soil in desertified areas is poor, the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N1/20C12R1/89C12R1/01
CPCC12N1/12C12N1/20
Inventor 杨新年段鹏程季祥
Owner 内蒙古阿尔格生命科学有限公司
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