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Application of bacteroides fragilis or zwitterionic capsular polysaccharide thereof in preparation of drugs for preventing and treating digestive system tumors

A technology of Bacteroides fragilis and capsular polysaccharide is applied in the field of biomedicine to achieve the effects of improving immune response, preventing and treating digestive system tumors, and reducing inflammatory response.

Active Publication Date: 2022-05-13
GUANGZHOU ZHIYI PHARMA INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, there is no report about the use of Bacteroides fragilis or its zwitterionic capsular polysaccharide as a probiotic for the treatment of digestive system tumors.

Method used

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  • Application of bacteroides fragilis or zwitterionic capsular polysaccharide thereof in preparation of drugs for preventing and treating digestive system tumors
  • Application of bacteroides fragilis or zwitterionic capsular polysaccharide thereof in preparation of drugs for preventing and treating digestive system tumors
  • Application of bacteroides fragilis or zwitterionic capsular polysaccharide thereof in preparation of drugs for preventing and treating digestive system tumors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Embodiment 1: the fermentation culture of Bacteroides fragilis

[0066] Streak inoculation of Bacteroides fragilis ZY-312 strain on blood plate, anaerobic culture for 48h. Observe the colony morphological characteristics, staining characteristics, size, club shape and distribution, etc.

[0067] Colony characteristics: Bacteroides fragilis ZY-312, after being cultured on a blood plate for 48 hours, is slightly convex, translucent, white, smooth, non-hemolytic, and the diameter of the colony is between 1-3mm. Seefigure 1 .

[0068] Morphology under the microscope: Bacteroides fragilis ZY-312 was examined by Gram staining. It is a Gram-negative bacterium with a typical rod shape, blunt rounded ends and dense staining. The uncolored part in the middle of the bacteria is like a vacuole. figure 2 .

[0069] Select a single colony and inoculate it in plant-derived peptone broth for 8 hours of fermentation (at a temperature of 37°C). The obtained bacterial liquid is centrif...

Embodiment 2

[0071] Example 2: Preparation of Bacteroides fragilis capsular polysaccharide

[0072] The bacteria slime prepared in Example 1 was used to carry out the experiment.

[0073] (1) Take 50g of bacteria slime, add 300g of purified water to resuspend the bacteria, adjust its pH to 3.5 with 1mol / L hydrochloric acid solution, extract at 100°C for 1.5h, cool to room temperature, centrifuge at 12000g at room temperature for 10min, take the supernatant to obtain rough sugar solution;

[0074] (2) The crude sugar solution is concentrated by ultrafiltration through a 10KD ultrafiltration membrane to remove small molecular impurities until the conductivity is stable, and the reflux liquid is collected;

[0075] (3) Add an equal volume of 40mmol / L Tris-HCl (pH8.5) to the reflux liquid to convert the salt; .5, containing 0.2mol / L NaCl) gradient elution for 25 column volumes, segmented collection, 100mL / bottle (component), SEC-HPLC tracking monitoring, combined 206nm absorption peak as a s...

Embodiment 3

[0077] Example 3 Bacteroides fragilis and its capsular polysaccharide prevent orthotopic transplantation of MFC gastric cancer in mice

[0078] 1. Experimental design and process

[0079] Preparation of gastric cancer orthotopic xenograft model:

[0080] (1) Using RPMI-1640 culture solution containing 10% calf serum under normal conditions (37°C, saturated humidity, 5% CO 2 ) to culture MFC digestive system tumor cells to the logarithmic growth phase, adjust the cell density to 1×10 7 individual / mL. Five male mice of 615 inbred strain were subcutaneously injected with 0.2 mL of MFC cell suspension in the right underarm under aseptic conditions, and the tumor-bearing mice were killed when the tumor grew to a diameter of 1 cm, and used as a tumor source for orthotopic transplantation.

[0081] (2) 90 male mice of 615 inbred strain were randomly divided into 9 groups according to body weight interval, namely blank group, model group, 5-fluorouracil group (5-FU, Sigma Company, ...

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Abstract

The invention discloses application of bacteroides fragilis and / or zwitterionic capsular polysaccharide thereof in preparation of drugs for preventing and treating digestive system tumors. A large number of experiments prove that the bacteroides fragilis ZY-312 with the preservation number of CGMCC No.10685 and the zwitterionic capsular polysaccharide thereof can relieve inflammatory response, protect normal tissues, improve anti-tumor immune response of organisms and effectively prevent and treat digestive system tumors.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of Bacteroides fragilis or its zwitterionic capsular polysaccharide in the preparation of drugs for preventing and treating digestive system tumors. Background technique [0002] According to the World Health Organization's 2018 global cancer statistics, half of the ten most common cancers in the world are digestive system tumors, and the incidence and mortality of colorectal cancer rank third and second among all malignant tumors, respectively. The mortality rate of systemic tumors and liver cancer is close behind. Chen et al.’s research on cancer incidence and mortality in China in 2014 showed that the incidence rates of digestive system tumors, colorectal cancer, and liver cancer are all ranked first in the incidence of malignant tumors in China. Five, and the mortality rates of liver cancer, digestive system tumors, esophageal cancer, colorectal cancer, and pancreat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/741A61K31/715A23L33/135A23C9/156A23C9/16A23C19/09A23G9/34A23G9/36A23K10/18A23K20/163A23L7/104A23L33/125A23C9/152A61P35/00C08B37/00
CPCA61K35/741A61K31/715A61P35/00C08B37/0003A23L33/135A23L33/125A23C9/16A23C9/156A23C9/152A23C19/09A23G9/363A23G9/34A23L7/104A23K10/18A23K20/163A23V2002/00A61K2300/00A23V2200/308A23V2200/314A23V2250/51Y02A50/30
Inventor 刘洋洋智发朝王晔李平
Owner GUANGZHOU ZHIYI PHARMA INC
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