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Lactobacillus fermentum LF028 with hypoglycemic effect and application thereof

A technology for fermenting Lactobacillus and lowering blood sugar, which is applied in the field of microbial engineering, and can solve problems such as rough taste, bitter or fishy smell, and severe powdery feeling, and achieve the effects of excellent flavor, uniform and delicate tissue, and strong water holding capacity

Active Publication Date: 2022-05-06
天津小薇生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, plant-based yogurt generally has problems such as rough taste, severe powdery feeling, bitter taste or fishy smell, w

Method used

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  • Lactobacillus fermentum LF028 with hypoglycemic effect and application thereof
  • Lactobacillus fermentum LF028 with hypoglycemic effect and application thereof
  • Lactobacillus fermentum LF028 with hypoglycemic effect and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 A strain of Lactobacillus fermentum LF028

[0038] Lactobacillus fermentum LF028 was isolated and screened from farmhouse sour soup in Guiyang City, Guizhou Province. The strain was deposited in the General Microbiology Center of China Committee for the Collection of Microbial Cultures on July 6, 2021. The preservation number is CGMCCNo. 22833.

[0039] Its 16S rDNA gene sequence is as follows:

[0040] ACTCTCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCGACTTCGTGCAGGCGAGTTGCAGCCTGCAGTCCGAACTGAGAACGGTTTTAAGAGATTTGCTTGCCCTCGCGAGTTCGCGACTCGTTGTACCGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATCTGACGTCGTCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCACTAGAGTGCCCAACTTAATGCTGGCAACTAGTAACAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACGACCATGCACCACCTGTCATTGCGTTCCCGAAGGAAACGCCCTATCTCTAGGGTTGGCGCAAGATGTCAAGACCTGGTAAGGTTCTTCGCGTAGCTTCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCAACCTTGCGGTCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTCCGGCAC...

Embodiment 2

[0041] Example 2 Experiment of acid resistance and bile salt resistance of Lactobacillus fermentum LF028

[0042] This example is an experiment on acid resistance and bile salt resistance of Lactobacillus fermentum LF028. The acid resistance experiment and bile salt resistance experiment of the strain were carried out respectively, including the following contents:

[0043] (1) Acid resistance test

[0044] S1. Preparation of artificial gastric juice

[0045] Take 10% hydrochloric acid and dilute with deionized water, adjust the pH to 2.0, 3.0, and 4.0 respectively, add pepsin to make the final concentration 1%, and then adjust the pH of the solution to 2.0, 3.0, and 4.0 with hydrochloric acid. In a sterile operating bench, filter with a 0.22 μm microporous membrane and set aside;

[0046] S2. Probiotic activation

[0047] S21. Take out the cryopreservation tube of Lactobacillus fermentum LF028 from the refrigerator, melt at room temperature, take 100 μL and inoculate it in...

Embodiment 3

[0069] Example 3 In vitro experiment on hypoglycemic effect of Lactobacillus fermentum LF028

[0070] This embodiment is an in vitro experiment on the hypoglycemic effect of Lactobacillus fermentum LF028, which includes the following contents:

[0071] (1) Preparation of fermentation broth and bacterial suspension

[0072] S1. Take out the cryopreservation tube of Lactobacillus fermentum LF028 from the refrigerator, dissolve it naturally, inoculate 100 μL into 10 mL of sterilized MRS medium, and incubate at 37°C for 24 hours to obtain the first-generation seed bacterial liquid; take the first-generation seed bacterial liquid with 3 % inoculum amount was inoculated into sterilized MRS medium, and the second-generation bacterial liquid was obtained after culturing at 37°C for 12 hours;

[0073] S2. Take 10 mL of the second-generation bacterial liquid, centrifuge at 8000 r / min for 10 min, and separate the bacterial cells and the fermentation supernatant;

[0074] S3. passing th...

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Abstract

The invention discloses lactobacillus fermentum LF028 with a hypoglycemic effect and application of the lactobacillus fermentum LF028. The lactobacillus fermentum LF028 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.22833; the invention also discloses an application of the lactobacillus fermentum LF028. The lactobacillus fermentum LF028 provided by the invention is a probiotic with a hypoglycemic effect and can be used for preparing a zymophyte agent, and the pure zymophyte agent can be used for further preparing fermented milk and plant yoghurt. The lactobacillus fermentum LF028 provided by the invention is a probiotic with a hypoglycemic effect, can be used for preparing a zymophyte agent, and is further applied to preparation of functional fermented milk and plant yoghurt.

Description

technical field [0001] The invention belongs to the field of microbial engineering and relates to a lactic acid bacterium, in particular to a lactobacillus fermentum LF028 with hypoglycemic effect and application thereof. Background technique [0002] According to the data released by IDF, there are 463 million people with diabetes in the world in 2019, and the number of people with diabetes will jump to 700 million by 2045. In my country, there are nearly 130 million people with diabetes, and about 200 million people with pre-diabetes. At present, the drug treatment of diabetes still faces challenges such as high price, high treatment cost and strong side effects. At present, the intervention of probiotics targeting intestinal flora has attracted more and more attention. Lactobacillus fermentum (Lactobacillus fermentum), as a typical probiotic, has multiple functions. Among them, the research on the hypoglycemic effect of Lactobacillus fermentum and the development of hea...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61P3/10A23C9/123A23C11/10A23L11/60A23L11/65A23L11/50A23L33/135C12R1/225
CPCC12N1/20A61P3/10A23C9/1234A23C11/10A23C11/106A23L11/60A23L11/65A23L11/50A23L33/135A23V2002/00A23V2400/143A23V2200/328
Inventor 韩清波刘瑞峰马乐辉李云旭
Owner 天津小薇生物科技有限公司
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