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A kind of shellfish yeast and its application

A technology of yeast and yeast freeze-dried powder, applied in the application, yeast-containing food ingredients, fungi and other directions, can solve the problem of low survival rate of bay yeast powder, and achieve good acid and bile salt resistance and tolerance. Strong, low-cost effect

Active Publication Date: 2020-08-04
北京中特养生物技术研究所有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The second technical problem to be solved by the present invention is the problem that the survival rate of Saccharomyces japonicus powder obtained by the freeze-dried bacterial powder process in the prior art is low, and then a kind of Saccharomyces japonicus freeze-dried powder with high survival rate of bacterial strains is provided. preparation method

Method used

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  • A kind of shellfish yeast and its application
  • A kind of shellfish yeast and its application
  • A kind of shellfish yeast and its application

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Effect test

Embodiment 1

[0043] The isolation and screening of embodiment 1 shellfish yeast

[0044] The Saccharomyces pumilii with the preservation number of the present invention being CGMCC No.16127 is isolated from human feces and obtained by the following method:

[0045] 1. The source of bacteria:

[0046] Put 1 gram of human intestinal feces into a sterilized bottle containing 30 glass balls and add physiological saline to make 100-fold dilution, fully beat evenly, and then make 10 -3 、10 -4 , to 10 in turn -8 Dilution, take 10 -4 、10 -5 、10 -6 、10 -7 、10 -8 Five dilutions of the bacterial solution were inoculated on LYT (self-made) universal agar plate, eosin, melanin agar, BB (BS) Bifidobacterium selective medium, LBS (LC) Lactobacillus selective medium, EC Enterococcus alternative and Sb yeast alternative. Each medium was connected to 6 plates, and the culture plate was turned up and down immediately after inoculation to spread the inoculum evenly on the surface of the plate. Three...

Embodiment 2

[0068] The preparation of embodiment 2 Saccharomyces japonicus freeze-dried powder

[0069] The Saccharomyces japonicus of this embodiment is a bacterial strain whose preservation number is CGMCC No.16127, and it is prepared as a Saccharomyces japonicus freeze-dried powder by the following method:

[0070](1) Get the bacterium liquid in the logarithmic growth phase obtained after the Saccharomyces japonicus is cultivated for 6h-12h, as the primary seed liquid, inoculate the primary seed liquid in the fermentation medium according to the inoculum size of 4-10%, Under the conditions of a temperature of 28-32°C and a rotational speed of 150-200rpm, cultivate for 24-36 hours to obtain a primary fermentation liquid; then, take the primary fermentation liquid and inoculate it into the fermentation culture according to the inoculum size of 4-10%. Under the conditions of temperature 28-32°C and rotating speed 100-180rpm, culture for 4-6h to obtain secondary fermentation liquid; then t...

Embodiment 3

[0074] The preparation of embodiment 3 shellfish lyophilized powder

[0075] The Saccharomyces japonicus of this embodiment is a bacterial strain whose preservation number is CGMCC No.16127, and it is prepared as a Saccharomyces japonicus freeze-dried powder by the following method:

[0076] (1) Take the Saccharomyces shellfish, and culture it in a fermentation medium at a temperature of 37° C. to obtain a fermentation liquid; wherein, the fermentation medium includes the following raw materials in parts by weight: 5 parts by weight of yeast extract powder, 10 parts by weight Parts by weight of peptone, 5 parts by weight of glucose, 5 parts by weight of sodium chloride, and 1 part by weight of trace salt;

[0077] (2) Get the fermented liquid obtained by fermentation in step (1), place it in a centrifuge with a rotating speed of 8000r / min, centrifuge for 30min, and collect the sludge;

[0078] (3) Add the protective agent to the fungus slime obtained in step (2), and the prot...

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Abstract

The present invention provides a shellfish yeast, which was submitted to the General Microbiology Center (CGMCC) of the China Microorganism Culture Collection Management Committee for preservation on July 17, 2018, and the preservation number is: CGMCC No.16127. The bay yeast provided by the invention has strong tolerance, good activity, can replace antibiotics, can adjust the balance of intestinal flora, and has a high survival rate after being prepared into freeze-dried powder.

Description

technical field [0001] The invention belongs to the technical field of human microecology, and in particular relates to a shellfish yeast and its application. Background technique [0002] With the deepening of people's understanding of antibiotics, antibiotics have been produced on a large scale and widely used. At the same time, people have paid more and more attention to the negative effects of antibiotics. Scientists have found that the abuse of antibiotics can cause normal bacteria in the body to Herd imbalance, the body develops drug resistance, and drug residues also threaten human health through animal products. Moreover, drug resistance can be transmitted to humans through bacteria through drug resistance factors (R factors), making health care and treatment more difficult, which will have a serious negative impact on human survival and development. As a result, many new drugs have emerged to replace antibiotics, among which probiotics are more effective. Probioti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12N1/02A23L33/14A61K36/064A61P37/04C12R1/85
CPCA23V2002/00A61K36/064A23L33/14A61P37/04C12N1/02C12N1/16C12N1/185C12R2001/85A23V2200/32A23V2250/76
Inventor 谭瑛
Owner 北京中特养生物技术研究所有限公司
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