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Dark energy-saving in-vitro propagation method for micro tubers of polygonatum cyrtonema

A Polygonatum multiflora, in vitro propagation technology, applied in the field of plant tissue culture, to achieve the effect of easy storage and high genetic stability

Active Publication Date: 2022-04-29
FUJIAN AGRI & FORESTRY UNIV
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Problems solved by technology

Studies have shown that in potato, patatin occurs in the early stage of tuber morphogenesis and is related to the formation of tubers. The Patatin protein family in Polygonatum has not been studied

Method used

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  • Dark energy-saving in-vitro propagation method for micro tubers of polygonatum cyrtonema
  • Dark energy-saving in-vitro propagation method for micro tubers of polygonatum cyrtonema
  • Dark energy-saving in-vitro propagation method for micro tubers of polygonatum cyrtonema

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Experimental program
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Embodiment 1

[0034] 1. Material method

[0035] In this study, Daodi Polygonatum multiflora tissue culture seedlings in Sanming City, Fujian Province were used as starting materials to optimize the subsequent medium and extract RNA. The sampling of the materials used in the experiment was repeated three times, and the samples were quickly frozen in liquid nitrogen. Store in -80°C refrigerator for later use.

[0036] 1.1 Induce the lateral buds of Polygonatum polyflora to form tubers:

[0037] (1) Establishment of Polygonatum multiflora aseptic system: remove the rotten part of Polygonatum multiflora fruit, wash and peel off the seeds with detergent, treat with 300mg / LGA3+300mg / L KT hormone, soak for 24h, add 75vol% alcohol to shake for 1min, and use Wash 3 times with sterile water, then disinfect with 0.1wt% mercuric chloride for 15 minutes, wash 3 times with sterile water, dry the water with sterile filter paper, and obtain sterile seeds;

[0038] (2) Germination of Polygonatum multiflo...

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Abstract

The invention provides a dark energy-saving in-vitro propagation method for micro tubers of polygonatum cyrtonema and belongs to the technical field of plant tissue culture. According to the method, the apical dominance of the explant is reduced by optimizing a plant growth regulator and carrying out in-vitro culture on the polygonatum kingianum explant, the growth potential of lateral buds is released from a dormant state, and tuber formation is induced. Cytology observation finds that the rosette type plant explant can form a plurality of tubers, and each tuber is derived from an axillary bud at the base of a leaf. Unlike adventitious organs, newly developed tubers originate from intrinsic lateral buds, and no callus is formed. The tuber production system of the present invention provides a new method for propagating polygonatum cyrtonema plants, facilitating easier storage, transplantation and tuber seeding. Under the condition that no callus is formed, higher hereditary stability can be obtained.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a dark energy-saving in vitro propagation method of Polygonatum multiflora microtubers. Background technique [0002] Polygonatum ( Polygonatum cyrtonema Hua) is the genus Polygonatum (Liliaceae) Polygonatum ) is a perennial herbaceous plant whose rhizomes are used as medicine. As a traditional Chinese medicine in my country, sealwort has been used for more than 2,000 years, and has the functions of nourishing qi, tonifying kidney, tonifying lung, and invigorating spleen. Polygonatum multiflora propagation methods are divided into rhizome propagation and seed propagation. The breeding cycle is long, and it usually reaches the harvest period in about 5 years. The germination time of seeds is longer, the germination rate is 60%-70%, the seed life is 2 years, and the seed reproduction cycle is longer, generally about 5 years; in production and cultivat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/002
Inventor 王姗姗班景洁林玉玲张城瑜王庆爽赖钟雄徐涵
Owner FUJIAN AGRI & FORESTRY UNIV
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