Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Culture medium for promoting germination of bletilla striata and preparation method thereof

A technology of culture medium and bletilla striata, applied in the field of culture medium, can solve the problems of long cultivation period, affecting the survival rate of seedling hardening cultivation, small protocorm, etc., and achieve the effects of promoting tissue differentiation, plant growth and rooting, and promoting division

Inactive Publication Date: 2022-04-22
安徽智野生物科技发展有限公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The related technology of bletilla striata tissue culture has been mature, but due to the long culture cycle, the protocorms cultivated are small, which affects the survival rate of seedling hardening and cultivation in the later stage

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A culture medium for promoting the germination of Bletilla striata, comprising the following raw materials in parts by weight: 50 parts of culture medium, 10 parts of organic liquid, 10 parts of macroelement base liquid, 1 part of growth-promoting substrate, and 50 parts of sterile water;

[0032] Wherein, the culture medium includes the following raw materials in parts by weight: MS medium, 200 parts of sucrose, 5 parts of NAA, 20 parts of 6Ba, 20 parts of adenine, and 5 parts of agar;

[0033] The organic liquid includes the following raw materials in parts by weight: 0.1 parts of vitamins, 20 parts of glycine, 50 parts of inositol, and 100 parts of coconut juice;

[0034] The macroelement base liquid includes the following raw materials in parts by weight: 2000 parts of potassium nitrate, 450 parts of ammonium sulfate, 150 parts of calcium chloride dihydrate, 150 parts of magnesium sulfate heptahydrate, and 300 parts of potassium dihydrogen phosphate;

[0035] The gr...

Embodiment 2

[0049] A culture medium for promoting the germination of Bletilla striata, comprising the following raw materials in parts by weight: 55 parts of culture medium, 12 parts of organic liquid, 12 parts of macroelement base liquid, 2 parts of growth-promoting substrate, and 60 parts of sterile water;

[0050] Wherein, the culture medium includes the following raw materials in parts by weight: MS medium, 300 parts of sucrose, 7.5 parts of NAA, 30 parts of 6Ba, 30 parts of adenine, and 7.5 parts of agar;

[0051] The organic liquid includes the following raw materials in parts by weight: 1.5 parts of vitamins, 30 parts of glycine, 75 parts of inositol, and 150 parts of coconut juice;

[0052] The macroelement base liquid includes the following raw materials in parts by weight: 3000 parts of potassium nitrate, 675 parts of ammonium sulfate, 225 parts of calcium chloride dihydrate, 225 parts of magnesium sulfate heptahydrate, and 450 parts of potassium dihydrogen phosphate;

[0053] T...

Embodiment 3

[0067] A culture medium for promoting the germination of Bletilla striata, comprising the following raw materials in parts by weight: 60 parts of culture medium, 15 parts of organic liquid, 15 parts of macroelement base liquid, 3 parts of growth-promoting substrate, and 70 parts of sterile water;

[0068] Wherein, the culture medium includes the following raw materials in parts by weight: MS medium, 400 parts of sucrose, 10 parts of NAA, 40 parts of 6Ba, 40 parts of adenine, and 10 parts of agar;

[0069] The organic liquid includes the following raw materials in parts by weight: 0.2 parts of vitamins, 40 parts of glycine, 100 parts of inositol, and 200 parts of coconut milk;

[0070] The macroelement base liquid includes the following raw materials in parts by weight: 4000 parts of potassium nitrate, 900 parts of ammonium sulfate, 300 parts of calcium chloride dihydrate, 300 parts of magnesium sulfate heptahydrate L, and 600 parts of potassium dihydrogen phosphate;

[0071] T...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a culture medium for promoting germination of bletilla striata and a preparation method thereof, and the preparation method comprises the following steps: step 1, slowly adding a culture matrix, an organic solution, a macroelement base solution and a growth-promoting substrate into sterile water, heating to 40-50 DEG C, and continuously stirring until the materials are completely and uniformly dissolved; 2, the dissolved substance is cooled, high-temperature sterilization is carried out, and the culture medium is obtained.The organic liquid is a compound of vitamin, glycine, inositol and coconut milk, the vitamin is prepared by compounding pyridoxine hydrochloride and nicotinic acid, and the vitamin and the glycine are added into the coconut milk; the bletilla striata extract can effectively participate in vital activities of protein metabolism, fat metabolism and sugar metabolism of cell tissues in a coenzyme form, and is matched with inositol to participate in carbohydrate metabolism and ion balance, so that the growth of bletilla striata tissues and the formation of embryoid and buds are effectively promoted.

Description

technical field [0001] The invention relates to the technical field of culture medium, in particular to a culture medium for promoting germination of bletilla striata and a preparation method thereof. Background technique [0002] Bletilla striata, also known as Baiji, is a perennial herb belonging to the family of Orchidaceae. The dried tubers are used for medicinal purposes, and have the effects of astringent, hemostasis, swelling and granulation. Clinically, it is often used to treat diseases such as tuberculosis hemoptysis, gastric ulcer hematemesis, blood in stool and traumatic bleeding. The tubers of Bletilla striata are rich in polysaccharides of Bletilla striata, the main component of which is glucomannan, which is widely used in food industry, medical and sanitary materials and daily chemical industry. [0003] Due to the high application value of Bletilla striata, the tissue culture technology of Bletilla striata has become a hot spot in current research. The re...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/002
Inventor 陈野牧闻智磊
Owner 安徽智野生物科技发展有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com