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Composite bionic body surface tissue and integrated construction method thereof

A construction method and body surface technology, applied in tissue regeneration, prosthesis, medical science, etc., can solve the problems of atrophy, blurred three-dimensional structure of the reconstructed auricle, deformation, etc., to improve the mechanical strength and meet the needs of clinical applications.

Active Publication Date: 2022-04-19
PLASTIC SURGERY HOSPITAL CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term follow-up found that the three-dimensional structure of the reconstructed auricle gradually blurred, atrophied, deformed and collapsed, and the long-term clinical effect did not meet expectations
Therefore, the insufficient mechanical strength of the tissue-engineered auricle has become a bottleneck restricting its clinical application.

Method used

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  • Composite bionic body surface tissue and integrated construction method thereof
  • Composite bionic body surface tissue and integrated construction method thereof
  • Composite bionic body surface tissue and integrated construction method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0053] Such as Figure 1 to Figure 12 As shown, this embodiment provides an integrated construction method of composite bionic body surface tissue, including the following specific steps:

[0054] S1: add an appropriate amount of auxiliary agent to the photosensitive gel of cartilage decellularized matrix and mix well to obtain the auxiliary agent-perichondrium bioink with a mass percentage of 10-50%;

[0055] S2: adding an appropriate amount of auxiliary agent 2 to the photosensitive gel of cartilage decellularized matrix and mixing to obtain a cartilage bioink with a mass percentage of auxiliary agent 2 of 10-50%;

[0056] S3: adding an appropriate amount of seed cells to the perichondrium bioink obtained in S1 to form a seed cell concentration of (1-50)×10 6 / mL of the mixed solution 1, while adding an appropriate amount of seed cells to the cartilage bio-ink obtained in S2 to form a seed cell concentration of (1 ~ 50) × 10 6 / mL of mixed solution II;

[0057] S4: Using ...

Embodiment 2

[0060] On the basis of Example 1, in this example, before S1, S0 is also included: pretreating the cartilage tissue to obtain a cartilage decellularized matrix photosensitive gel. Simple preparation process, providing biomimetic raw material cartilage acellular matrix photosensitive gel for perichondrium and cartilage bioink

Embodiment 3

[0062] On the basis of Example 2, in this example, the cartilage tissue in the S0 is one or more of ear cartilage, articular cartilage, costal cartilage, scapular cartilage, and meniscus. The method is convenient to obtain, and is beneficial to the preparation of bionic reconstructed body surface tissue.

[0063] It should be noted that the above-mentioned cartilage tissue is usually pig cartilage tissue, which can be purchased in the market and can be industrialized.

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Abstract

The invention relates to a composite bionic body surface tissue and an integrated construction method thereof, and the method comprises the following specific steps: adding a proper amount of an auxiliary agent into cartilage acellular matrix photosensitive gel, and uniformly mixing to obtain cartilage membrane bio-ink; adding a proper amount of auxiliary agent II into the cartilage acellular matrix photosensitive gel, and uniformly mixing to obtain cartilage biological ink; adding seed cells into cartilage membrane bio-ink to form a mixed solution I, and adding a proper amount of seed cells into cartilage bio-ink to form a mixed solution II; and integrally constructing the composite bionic body surface tissue by using the mixed solution I and the mixed solution II. The method has the beneficial effects that the body surface tissue with the bilateral cartilage membranes and the middle cartilage tissue is constructed, the structure and composition characteristics of the body surface tissue containing the cartilage membranes are simulated, and different bionic scaffold materials and functional cells are utilized to realize mechanical bionics through structure and composition bionics, so that the mechanical strength of the construction material is improved, and the construction cost is reduced. The clinical application requirements of body surface tissue reconstruction are met.

Description

technical field [0001] The invention relates to the technical field of three-dimensional bioprinting and tissue and organ repair and reconstruction, in particular to a composite bionic body surface tissue and an integrated construction method thereof. Background technique [0002] Congenital microtia is the second largest craniofacial congenital deformity in my country, which seriously affects the physical and mental health of children. At present, the most effective treatment method is auricle reconstruction based on autologous costal cartilage carving, which is difficult, risky and has many postoperative complications. The rapid development of tissue engineering and regenerative medicine technology has brought new strategies for auricle reconstruction. At present, based on cartilage tissue engineering technology, polyglycolic acid / polylactic acid (PGA / PLA) and chondrocytes have successfully achieved the first international clinical breakthrough of tissue engineering auric...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/38A61L27/20A61L27/22A61L27/52B33Y10/00B33Y70/10B33Y80/00
CPCA61L27/3612A61L27/3687A61L27/3691A61L27/222A61L27/227A61L27/20A61L27/3804A61L27/3834A61L27/52B33Y70/10B33Y10/00B33Y80/00A61L2430/14
Inventor 蒋海越刘霞贾立涛
Owner PLASTIC SURGERY HOSPITAL CHINESE ACAD OF MEDICAL SCI
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