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Homogeneous immunodetection kit for detecting 14-3-3 eta protein and application of homogeneous immunodetection kit

An immunodetection method and protein technology, applied in the field of immunoassay, can solve the problems of low accuracy, cumbersome operation, complicated operation, etc., and achieve the effect of wide signal value range, wide linear range and good stability.

Pending Publication Date: 2022-04-15
CHEMCLIN DIAGNOSTICS CO LTD
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] Existing methods for detecting 14-3-3 protein are heterogeneous immunoassays, which are less sensitive, less accurate, and more complicated to operate
Moreover, no commercial 14-3-3eta protein detection kit has been found in the domestic and foreign markets
It has been reported that enzyme-linked immunosorbent assay (ELISA) was used to detect 14-3-3eta protein in serum of patients, but its sensitivity is low, specificity is poor, and the operation is relatively cumbersome.

Method used

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  • Homogeneous immunodetection kit for detecting 14-3-3 eta protein and application of homogeneous immunodetection kit

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Embodiment approach

[0087] As mentioned above, the existing methods for detecting 14-3-3 protein are all heterogeneous immunoassay methods, which have low sensitivity, low accuracy and complicated operation. The inventors found that the detection of 14-3-3 protein by homogeneous immunoassay has high sensitivity, wide linear range and good precision, and the detection process does not require steps such as washing, and the operation is relatively simple.

[0088] Therefore, the first aspect of the present invention relates to the detection of the presence of 14-3-3eta protein or fragments thereof or the presence of immune complexes formed by said 14-3-3eta protein or fragments thereof and at least one antibody in the preparation of a homogeneous Immunoassay method for use in a reagent for assessing rheumatoid arthritis in a subject by: a) providing a test sample from a subject suspected of having rheumatoid arthritis; b) detecting said test 14-3-3eta protein or its fragment or the immune complex f...

Embodiment 1

[0216] 1. Preparation of reagent Ⅰ (antibody-coated receptor):

[0217] (1) Preparation of acceptor and donor

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Abstract

The invention relates to a homogeneous immunodetection kit for detecting a 14-3-3eta protein and a preparation method of the homogeneous immunodetection kit. The kit is prepared from a first antibody and a second antibody which can be specifically combined with 14-3-3eta protein, in a double-antibody sandwich mode, by utilizing the advantages of high sensitivity, wide linear range, good precision and the like of a light-activated chemiluminescence immunoassay platform, the definite diagnosis rate is increased for clinical diagnosis of rheumatoid, and auxiliary detection is provided for preventing rheumatoid and finding rheumatoid in advance.

Description

[0001] This application is a divisional application of a Chinese patent application with an application date of January 5, 2018, an application number of 201810011520.0, and an invention title of "Homogeneous Immunoassay Kit for Detecting 14-3-3eta Protein and Its Application". technical field [0002] The invention belongs to the technical field of immune analysis, and in particular relates to a homogeneous immunoassay kit for detecting 14-3-3 eta protein, a preparation method and a use method thereof. Background technique [0003] 14-3-3eta(η) protein is a novel serum / plasma protein marker that induces inflammatory factors such as interleukin (IL)-1 and -6 and is linked to joint damage. Among the seven subtypes, only 14-3-3η protein was highly expressed in RA active inflammatory synovium and serum, and was positively correlated with the expression of RA pro-inflammatory factors and synovial inflammation, and the level of 14-3-3η protein in synovial fluid At least 5 times h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/558
CPCG01N33/54313G01N33/558
Inventor 饶星陈英豪刘宇卉李临
Owner CHEMCLIN DIAGNOSTICS CO LTD
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