Vacuum bag type rumen in-vitro fermentation device and operation method thereof

Abstract

The invention discloses a vacuum bag type rumen in-vitro fermentation device and an operation method thereof. The vacuum bag type rumen in-vitro fermentation device comprises a water bath constant-temperature oscillator, a fermentation bag, an F57 cellulose filter bag, a 00 # silica gel plug, 20 mm punched glass beads, a nylon rope, a plane bag vacuum packaging machine, a 200 mL injector with scales and a pulse heat sealing machine. The method comprises the following steps: collecting rumen fluid; adding sodium erythorbate into the buffer solution to remove oxygen until the buffer solution becomes brown yellow; a fermentation suction nozzle bag is matched with a cellulose filter bag for fermentation, an inclined opening of the suction nozzle bag is sealed, an opening is vacuumized and deoxygenized and sealed, and rumen in-vitro fermentation is simulated in a constant-temperature oscillator. When the system is used for rumen in-vitro fermentation, the measurement result is stable and accurate, simplicity and convenience are realized, and the cost is low.

Description

technical field [0001] The invention relates to the technical field of rumen in vitro fermentation for ruminants, in particular to a vacuum bag type rumen in vitro fermentation device and an operating method thereof. Background technique [0002] As a microbial fermenter, the rumen provides more than 70% of the body's energy requirements for ruminants, and is the focus of ruminant nutrition research. The technology of simulating rumen fermentation in vitro has the advantages of short test period, high repetition number, and not being limited by live animal tests. It is an important means to evaluate rumen function and forage quality. Evaluation and other fields are widely used. [0003] At present, the method of simulating rumen fermentation in vitro mainly uses fermentation bottles or syringes, and injects saturated CO 2 Reach anaerobic conditions and realize the simulation of rumen anaerobic fermentation conditions. These methods have at least the following deficiencies...

AI Technical Summary

Problems solved by technology

[0008] The main purpose of the present invention is to provide a rumen in vitro fermentation system and operation method with low cost and stable data collection. By combining the basic principle of in vitro rumen simulation fermentation, vacuum bag system and chemical deoxygenation method, it overcomes the existing equipment Due to the defects of high cost, complex operation and insufficient stability of collected data, a vacuum bag type rumen in vitro fermentation device with low cost and stable collected data and its operating method and its operating method were designed.

Images