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Application of febrifugine B in improving killing power of NK (Natural Killer) cells

A technology of NK cells and hibisole, which is applied in the field of cell therapy, can solve problems such as the influence of hibisole, which has not been published in research, and achieve the effect of improving the proliferation activity and lethality of NK cells and improving the lethality

Inactive Publication Date: 2022-04-01
淮安泰凯睿医药科技有限公司
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  • Abstract
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  • Claims
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Problems solved by technology

However, there are no studies disclosing the effects of ethosine on other immune cells such as NK cells

Method used

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  • Application of febrifugine B in improving killing power of NK (Natural Killer) cells
  • Application of febrifugine B in improving killing power of NK (Natural Killer) cells
  • Application of febrifugine B in improving killing power of NK (Natural Killer) cells

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Embodiment Construction

[0013] The substantive content of the present invention will be specifically described below in conjunction with the embodiments, but those skilled in the art should know that the protection scope of the present invention should not be limited to the specific embodiments.

[0014] 1. Experimental materials

[0015] NK cell culture medium was purchased from CellGro SCGM, Germany, and fetal bovine serum and RPMI 1640 culture medium were purchased from Gibco. K562 was purchased from ATCC. ELISA kits were purchased from Shanghai Renjie Biotechnology Co., Ltd. Changshan B was purchased from Chengdu Ruifensi Biotechnology Co., Ltd., with a purity of 98%. rhIL-2 and gentamicin were purchased from Suzhou Renode Biotechnology Co., Ltd.

[0016] 2. Experimental method

[0017] 1. NK cell culture

[0018] The peripheral blood of healthy volunteers was collected, Ficoll density gradient centrifugation, and peripheral blood mononuclear cells were separated, and the peripheral blood wa...

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Abstract

The invention discloses an application of febrifugine B in improving the killing power of NK (Natural Killer) cells. Based on a cell proliferation experiment, a Western blot experiment, an ELISA experiment and a target cell killing experiment, it is found that the febrifugine B can promote NK cell proliferation and improve the killing power of the NK cells in vitro, which shows that the febrifugine B has the effect of improving the proliferation activity and the killing power of the NK cells and can be used for developing a culture medium for improving the proliferation activity and the killing power of the NK cells.

Description

technical field [0001] The invention belongs to the field of cell therapy, and relates to the cultivation of NK cells with high lethality, in particular to the use of echinacein for improving the lethality of NK cells. Background technique [0002] NK cells are an important part of innate immunity. The anti-tumor effect of NK cells is not only reflected in the killing of tumor cells, but also associated with immune cells such as DCs in the tumor microenvironment, which can strengthen the adaptive immune response. In addition, NK cells can target and kill tumor stem cells and play an important role in anti-tumor metastasis. NK cells can directly kill tumor cells. After NK cells activate and recognize target cells through the synergistic effect of surface inhibitory receptors and activating receptors, synapses will be established with target cells to deliver granzymes and perforin, which can be inserted into the plasma of target cells. membrane and form holes that lead to os...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0783
Inventor 不公告发明人
Owner 淮安泰凯睿医药科技有限公司
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