An easy-to-use porous brain slice incubation device

Abstract

The invention relates to a convenient-to-use porous brain slice incubation device, which comprises an incubation device body; an incubation chamber and an oxygen passage are arranged in the incubation device body, the oxygen passage communicates with the incubation chamber, and the oxygen passage connects an oxygen supply pipeline; the interior of the incubation chamber The incubation board is set, and the activity of the incubation board is set inside the incubation chamber; the incubation board is provided with at least one incubation hole, and an incubation net for carrying brain slices / tissue slices is arranged under the incubation hole; the incubation room is set to divide the incubation room into different spaces. Components; a temperature control device is installed outside the incubation device body, and the temperature control device still uses the principle of the water bath method; because incubation under water bath conditions can maintain the temperature well, the temperature is controlled and heated evenly; in this way, the brain slices / tissues The slice is placed in the incubation hole, and the oxygen supply pipeline and oxygen channel are used to provide oxygen to the brain slice / tissue slice and incubate; setting up the spacer can effectively avoid wasting the incubation solution when incubating a single brain slice.

Description

technical field [0001] The invention belongs to the technical field of medical devices, and in particular relates to a convenient-to-use porous brain slice incubation device. Background technique [0002] Brain slice is a commonly used specimen in current neuroscience research. The method is to remove the brain tissue or spinal cord from the animal body and cut it into 200-500μm thin slices in artificial cerebrospinal fluid. These flakes retain the activity and function of part of the neural tissue, compared with discrete or cultured cells, they have the advantage of retaining a relatively complete neural circuit and extracellular environment; compared with in vivo, the influencing factors are relatively simple and more predictable When preparing brain slices, multiple brain slices are usually cut out for the brain region of interest, and then the brain slices are placed in artificial cerebrospinal fluid for incubation. The common method is to place all the brain slices in a...

AI Technical Summary

Problems solved by technology

[0004] Aiming at the above problems, the present invention provides a convenient-to-use porous brain slice incubation device, which effectively ensures a suitable incubation environment, and at the same time solves the problem that multiple incubations can be incubated together, and single incubation can effectively save the incubation solution.

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