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Fermentation medium for Cap protein virus-like particles of porcine circovirus type 2

A type 2 virus, protein virus technology, applied in the direction of fermentation, virus, viral peptides, etc., can solve the problems of increased production cost, many miscellaneous proteins, low product yield, etc., to save production cost, improve production efficiency, and reduce purification. effect of difficulty

Pending Publication Date: 2022-03-29
成都史纪生物制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, this method faces the following problems: 1. The product yield is low; 2. There are more miscellaneous proteins (proteins other than Cap protein) produced, which puts higher requirements on the purification platform process and increases the production cost

Method used

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  • Fermentation medium for Cap protein virus-like particles of porcine circovirus type 2
  • Fermentation medium for Cap protein virus-like particles of porcine circovirus type 2
  • Fermentation medium for Cap protein virus-like particles of porcine circovirus type 2

Examples

Experimental program
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Embodiment 1

[0040] Embodiment 1 Fermentation method of the present invention

[0041] 1. Strains

[0042] The bacterial strain is Escherichia coli (Chaperone competent cells pGro7 / BL21(DE3)), and the bacterial strain includes the expression vector pET28a carrying the target gene (gene expressing Cap protein), the sequence of the target gene is shown in SEQ ID NO.1, Located at the XhoI (restriction site) position of the vector.

[0043] SEQ ID NO.1:

[0044] atgacctacccgcgtcgtcgtttccgtcgtcgtcgtcaccgtccgcgttctcacctgggt 60

[0045] cagatcctgcgtcgtcgtccgtggctggttcacccgcgtcaccgttaccgttggcgtcgt 120

[0046] aaaaacggtatcttcaacacccgtctgtctcgtaccatcggttacaccgttaaaaaaacc 180

[0047] accgttcgtaccccgtcttggaacgttgacatgatgcgtttcaacatcaacgacttcctg 240

[0048] ccgccgggtggtggttctaacccgctgaccgttccgttcgaatactaccgtatccgtaaa 300

[0049] gttaaagttgaattctggccgtgctctccgatcacccagggtgaccgtggtgttggttct 360

[0050]accgctgttatcctggacgacaacttcgttaccaaagctaacgctctgacctacgacccg 420

[0051] tacgttaactactcttc...

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Abstract

The invention discloses a fermentation medium for porcine circovirus type 2 Cap protein virus-like particles (Cap-VLP), which comprises the following components in parts by weight: 7 to 13 parts of tryptone, 7 to 13 parts of yeast extract, 7 to 13 parts of sodium chloride, 7 to 13 parts of glycerol, 15 to 25 parts of disodium hydrogen phosphate, 6 to 12 parts of monopotassium phosphate and 4 to 8 parts of magnesium sulfate. The additive comprises the following components in parts by weight: 3-9 parts of ammonium sulfate, 1-4 parts of copper sulfate, 1-4 parts of cobalt chloride and 0.001-0.002 part of biotin. The invention also discloses a method for fermenting Cap protein virus-like particles by using the culture medium. The culture medium and the method do not need a purification step, and are more efficient compared with a traditional method.

Description

technical field [0001] The invention belongs to the technical field of fermentation, and in particular relates to a fermentation medium of porcine circovirus type 2 virus Cap protein virus-like particles and a fermentation method thereof. Background technique [0002] Porcine circovirus (Porcine circovirus, PCV) is one of the smallest known animal viruses. The virus can infect the lymphoid tissue of pigs and lead to the suppression of the immune system; especially young weaned piglets can cause post-weaning multi-systemic wasting syndrome (Post-weaning mutil-systemic wasting syndrome, PMWS). Weaned piglets suffering from PMWS will continue to produce inflammatory responses due to the interference of T lymphocytes, which are one of the target cells for PCV2 virus replication. Because PCV2 invades the lymphoid tissue of pigs and continues to replicate in the cells, the number of white blood cells is continuously reduced, the immune ability of the body is destroyed, and the im...

Claims

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Application Information

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IPC IPC(8): C12P21/02C12N15/70C07K14/01C12R1/19
CPCC12P21/02C12N15/70C07K14/005C12N2750/10023
Inventor 岳丰雄刘汉平邢刚宋攀潘倩林艳
Owner 成都史纪生物制药有限公司
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