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Ligand use cost evaluation method based on unit protein adsorption cost

A protein and ligand technology, applied in the field of protein chromatography separation, can solve the problems of infeasibility, increase in the cost of adsorbent preparation, and decrease in adsorption capacity, etc., and achieve the effect of high industrialization potential, good guiding significance, and small workload

Inactive Publication Date: 2022-03-25
TAIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, many studies have shown that there is a complex interaction between adsorption performance and ligand density. High ligand density does not necessarily improve the performance of the adsorbent, and even reduces the adsorption capacity due to excessive steric hindrance, which also means that The increase in the cost of adsorbent preparation
Therefore, it is neither economical nor feasible to improve the adsorption performance simply by increasing the ligand density.

Method used

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  • Ligand use cost evaluation method based on unit protein adsorption cost
  • Ligand use cost evaluation method based on unit protein adsorption cost
  • Ligand use cost evaluation method based on unit protein adsorption cost

Examples

Experimental program
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Effect test

Embodiment 1

[0042] Such as Figure 1-6 As shown, the evaluation method includes the following steps:

[0043] S1. Obtain ligands to be evaluated and ligand information.

[0044] The ligand to be evaluated is a hydrophobic charge-inducing ligand, and tryptophan ligand is used in this example. Obtain basic information of tryptophan ligands from suppliers, including molecular weight, brand, unit price, etc.

[0045] S2. Coupling the ligand to be evaluated to the target substrate, and calculating the coupling efficiency of the ligand to be evaluated.

[0046] The target substrate is a hydrophilic medium with a porous structure and surface hydroxyl groups. In this embodiment, the target matrix is ​​agarose gel microspheres. The basic information of matrix and tryptophan ligand in the present embodiment is as shown in table 1:

[0047] Table 1

[0048]

[0049] 4% agarose gel microspheres were taken for coupling reaction to prepare hydrophobic charge-induced adsorbents with different l...

Embodiment 2

[0068] Obtain basic information of tryptamine ligands from suppliers, including molecular weight, brand, unit price, etc. 4% agarose gel microspheres were taken for coupling reaction to prepare hydrophobic charge-induced adsorbents with different ligand densities. The specific preparation process is as follows: the drained cross-linked agarose matrix is ​​first activated by allyl bromide, and then brominated by N-bromosuccinimide. By controlling the amount of ligand added in the coupling reaction, different ligand densities can be obtained. Finally, the prepared adsorbents were washed with 0.1 M HCl, 0.1 M NaOH, and deionized water, respectively. Referring to Example 1, perform data processing and calculation to obtain molecular weight, unit price, coupling efficiency, specific static saturation adsorption capacity or specific dynamic adsorption capacity (10% breakthrough), and calculate the unit protein adsorption cost under static adsorption and dynamic adsorption condition...

Embodiment 3

[0071] Obtain the basic information of 2-(aminomethyl)pyridinium from the supplier, including molecular weight, brand, unit price, etc. 4% agarose gel microspheres were taken for coupling reaction to prepare hydrophobic charge-induced adsorbents with different ligand densities. The specific preparation process is as follows: the drained cross-linked agarose matrix is ​​first activated by allyl bromide, and then brominated by N-bromosuccinimide. By controlling the amount of ligand added in the coupling reaction, different ligand densities can be obtained. Finally, the prepared adsorbents were washed with 0.1 M HCl, 0.1 M NaOH, and deionized water, respectively. Referring to Example 1, perform data processing and calculation to obtain molecular weight, unit price, coupling efficiency, specific static saturation adsorption capacity or specific dynamic adsorption capacity (10% breakthrough), and calculate the unit protein adsorption cost under static adsorption and dynamic adsorp...

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Abstract

The invention discloses a unit protein adsorption cost-based ligand use cost assessment method. The method comprises the following steps: acquiring a ligand to be assessed and ligand information; coupling the ligand to be evaluated to the target matrix, and calculating the coupling efficiency of the ligand to be evaluated; performing a static adsorption experiment on the to-be-evaluated ligand, and calculating the specific static saturation adsorption capacity of the to-be-evaluated ligand; carrying out a penetration experiment on the to-be-evaluated ligand, and calculating the specific dynamic adsorption capacity of the to-be-evaluated ligand; calculating the unit protein adsorption cost under the static adsorption condition based on the ligand information, the coupling efficiency and the specific static saturated adsorption capacity, and calculating the unit protein adsorption cost under the dynamic adsorption condition based on the ligand information, the coupling efficiency and the specific dynamic adsorption capacity; based on the unit protein adsorption cost under the static adsorption condition and the unit protein adsorption cost under the dynamic adsorption condition, the influence of the ligand type and density on the use cost is evaluated. The method can effectively help optimization of ligand types and densities in the industrial preparation process of the adsorbent.

Description

technical field [0001] The invention relates to the field of protein chromatography separation, in particular to a ligand usage cost evaluation method based on unit protein adsorption cost. Background technique [0002] A ligand is a functional molecule used in the preparation of an adsorbent. The performance of the adsorbent is mainly determined by the ligand together with several other parameters (such as matrix particle size, pore size, etc.). Ligand is one of the most influential parameters and therefore deserves a lot of attention. But in recent years, thanks to the development of materials science, there are relatively more researches on new matrix materials, but relatively few studies on ligands. Generally speaking, the research on these ligands can be divided into two categories: 1. Focus on the discovery and design of new ligands, such as benzotriazole-5-carboxyl, polypeptide Ac-PHQGQHIGVSK and so on. The problem is that the discovery of new efficient ligands is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06Q10/06G06Q50/04G16B15/00G16B40/00G01N15/08G01N33/00
CPCG06Q10/06313G06Q10/0639G06Q50/04G16B15/00G16B40/00G01N15/08G01N33/00Y02P90/30
Inventor 施伟朱舜囡许虹孔莘雅付佳雪章小斌厉凯彬张思奇韩得满
Owner TAIZHOU UNIV
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