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Kluyveromyces marxianus high-density fermentation culture medium and use method thereof

A technology of high-density fermentation and Kluyveromyces, which is applied in the field of high-density fermentation medium of Kluyveromyces marx, can solve the problem of low concentration of fermentation bacteria and achieve high-quality industrial production transformation and low cost

Active Publication Date: 2022-03-22
WUHAN SUNHY BIOLOGICAL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to overcome the generally low concentration of Kluyveromyces marx fermentation cells in the prior art

Method used

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  • Kluyveromyces marxianus high-density fermentation culture medium and use method thereof
  • Kluyveromyces marxianus high-density fermentation culture medium and use method thereof

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Embodiment 1

[0039] The Kluyveromyces marx high-density fermentation method adopted in the present embodiment comprises the following steps:

[0040] (1) Strain activation:

[0041] Inoculate Kluyveromyces marxe into solid medium for activation, the activation temperature is 30°C, and the activation time is 60h to obtain purified strains; inoculate the purified strains into YPD medium, culture at 30°C for 35h, and set aside;

[0042] (2) Preparation of seed solution:

[0043] The activated Kluyveromyces marx strain was inoculated into the shake flask seed liquid medium for fermentation culture, the culture temperature was 30°C, the shaker speed was 150rpm / min, and the culture time was 20h to obtain the first-grade seed liquid;

[0044] Inoculate the first-grade seed liquid into the seed fermentation tank with 8% inoculation amount for fermentation and cultivation. The cultivation temperature is 30°C, the rotation speed is 200-400rpm / min, the tank pressure is 0.03-0.05Mpa, and the pH is ad...

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Abstract

The invention belongs to the technical field of bioengineering, and particularly provides a safe and efficient Kluyveromyces marxianus high-density fermentation medium and a use method thereof, optimal fermentation conditions are obtained by optimizing a fermentation process, and by adopting the method provided by the invention, the fermentation efficiency is improved, and the fermentation cost is reduced. The feeding speed is adjusted, so that the viable count of the Kluyveromyces marxianus fermentation tank can be remarkably increased, the concentration of bacteria in the final fermentation liquid in the tank can reach about 1.2 * 10 < 10 > cfu / mL, the problem that the viable count of the yeast in the fermentation liquid in the tank is generally low in the existing Kluyveromyces marxianus fermentation process is solved, and high-quality industrial production conversion is realized.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a high-density fermentation medium of Kluyveromyces marxe and a use method thereof. Background technique [0002] Kluyveromyces kluyveromyces belongs to the genus Kluyveromyces of the family Saccharomyces, and is widely found in natural environments such as kumiss, milk, and fruits. Kluyveromyces maximus is a food-safe yeast whose safety in the food and medical industries has been confirmed by the Ministry of Health of China, the European Food Safety Administration, and the US Food and Drug Administration, that is, it can be eaten by humans. As a new type of yeast, Kluyveromyces marx also has its application potential in feed. In Europe, the Food Safety Administration has included Kluyveromyces marx in the scope of safe biological agents added to food and feed, indicating the safety and important value of Kluyveromyces marx. In China, researchers have recogniz...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12N1/38C12R1/645
CPCC12N1/16C12N1/38
Inventor 陈新伟申进军张成杰周樱詹志春
Owner WUHAN SUNHY BIOLOGICAL
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