Preparation method of collagen active peptide for inhibiting cancer cell proliferation

Abstract

The invention discloses a preparation method of collagen active peptide for inhibiting cancer cell proliferation, relates to the technical field of protein and polypeptide preparation, and particularly relates to 80-100 parts of bone collagen peptide, 5-15 parts of bovine bone marrow peptide, 1-3 parts of inulin, 0.5-1.5 parts of acerola cherry fruit powder, 10-30 parts of taurine, 5-15 parts of vitamin B1, 5-10 parts of vitamin B2 and 2-4 parts of vitamin B6. According to the preparation method of the collagen active peptide for inhibiting cancer cell proliferation, micro-vibration with the vibration amplitude within 0.5 cm is carried out on a material disc containing materials in the freeze-drying process, so that a solution continuously fluctuates under the vibration effect, the solution is subjected to freeze-drying in the fluctuating process, the disintegration phenomenon is solved, and the cancer cell proliferation is inhibited. A closed escape passage of water vapor at the lower layer is avoided, so that the control requirement on the disintegration temperature is reduced, the drying speed of the solution is indirectly accelerated, the solution is limited by the molecular film when fluctuating and is prevented from falling out of the material disc, and meanwhile, escape of the water vapor is not hindered by the molecular film.

Description

technical field [0001] The invention relates to the technical field of protein and polypeptide preparation, in particular to a method for preparing collagen active peptides that inhibit cancer cell proliferation. Background technique [0002] Food-derived bioactive peptides represent a class of resources that can promote human health. Since these active peptides can be absorbed during gastrointestinal digestion and become active, they provide new applications for the prevention or treatment of chronic diseases. In recent years, peptides It has been recognized to have biological activities, such as regulating immunity, anti-microbial, anti-hypertensive, anti-thrombotic, anti-cancer, anti-oxidation and cholesterol-lowering activities. These active peptides are derived from milk, wheat, soybean, egg, fish protein and other plants And animals, the active polypeptide needs to be freeze-dried in the later stage of preparation to change from liquid to solid, so as to remove water a...

AI Technical Summary

Problems solved by technology

[0004] Aiming at the deficiencies of the prior art, the present invention provides a preparation method of collagen active peptides that inhibit the proliferation of cancer cells, which solves the problem that the existing active peptides need to maintain the temperature before disintegration during freeze-drying in the above-mentioned background technology. Below the temperature, to avoid the disintegration phenomenon of the active peptide similar to collapse, to avoid closing the escape path of the active peptide water vapor in the lower layer, but the temperature is kept at the disintegration temperature, resulting in the problem that the efficiency of freeze-drying cannot be further improved