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Surface modification method of plastic centrifugal tube for extracting biological sample

A technology for biological samples and centrifuge tubes, applied in the coating and other directions, can solve the problems of inability to ensure protein molecules, poor surface modification uniformity, and dead corners of centrifuge tube modification, and achieve good dipping treatment effect, high uniformity, and low Adsorbent effect

Active Publication Date: 2022-03-18
赛宁(苏州)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, when the technical solutions provided in the above-mentioned prior art are applied to centrifuge tubes with low aspect ratio and large diameter, relatively uniform surface modification can be obtained effect, but when the above-mentioned technical solution is applied to a centrifuge tube with a high aspect ratio and a small diameter, especially for a centrifuge tube with an aspect ratio greater than 10 and a diameter less than 7mm, the centrifuge tube will be hindered by the remaining gas at the bottom of the centrifuge tube. There are dead angles in the modification of centrifuge tubes, resulting in poor uniformity of surface modification, which cannot ensure low adsorption of protein molecules on all surfaces during use

Method used

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  • Surface modification method of plastic centrifugal tube for extracting biological sample
  • Surface modification method of plastic centrifugal tube for extracting biological sample
  • Surface modification method of plastic centrifugal tube for extracting biological sample

Examples

Experimental program
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Effect test

Embodiment 1

[0058] A method for surface modification of plastic centrifuge tubes for extracting biological samples, said method comprising the steps of:

[0059] Degreasing and cleaning the plastic centrifuge tube, the plastic centrifuge tube is made of PP material, the length is 8cm, and the maximum diameter is 6mm;

[0060] Prepare an acetone solution containing 2.5-3wt% benzophenone as a hydroxylation solution, and add the hydroxylation solution to figure 1 The impregnation chamber 1 of the hydroxylation equipment shown, the plastic centrifuge tube is placed on the rotating fixture 10 and placed on the mounting seat 11 in the impregnation chamber 1 and immersed in the hydroxylation solution for hydroxylation reaction, and the installation is driven by the rotating device 12 Seat 11 rotates intermittently; rotate forward at a speed of 10 rpm for 4 minutes, then stop the rotation and make the opening of the plastic centrifuge tube downward for 40 seconds, then continue to rotate in the o...

Embodiment 2

[0064] The surface modification method of a plastic centrifuge tube for extracting biological samples provided in this example is basically the same as that in Example 1, the difference is that:

[0065] When carrying out hydroxylation treatment, also use figure 1 The shown equipment pressurizes the reaction system, and the applied air pressure is 8kPa greater than the external atmospheric pressure.

Embodiment 3

[0067] The surface modification method of a plastic centrifuge tube for extracting biological samples provided in this example is basically the same as that in Example 1, the difference is that:

[0068] During the hydroxylation treatment, a stepping device is used to control the plastic centrifuge tube to reciprocate linearly in the hydroxylation solution along the length direction of the rotating fixture 10. The moving distance is 18mm, and the time for one reciprocating movement is 12s.

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Abstract

The invention discloses a surface modification method of a plastic centrifugal tube for extracting a biological sample. The surface modification method comprises the following steps: dipping a plastic centrifuge tube in a hydroxylation solution, intermittently rotating the plastic centrifuge tube around an axis perpendicular to the gravity direction so as to realize hydroxylation treatment on the plastic centrifuge tube, and then rinsing, drying and performing ultraviolet irradiation, and then at least coating the inner wall of the plastic centrifugal tube subjected to hydroxylation treatment with a modified solution, and carrying out drying and heat treatment to obtain the low-protein adsorption plastic centrifugal tube. According to the surface modification method provided by the invention, the hydroxylation treatment uniformity of the centrifugal tube with high length-diameter ratio and small diameter is ensured, and the low adsorbability of the surface of the centrifugal tube subjected to surface modification treatment is further ensured.

Description

technical field [0001] The invention relates to the technical field of surface modification, in particular to a method for surface modification of a plastic centrifuge tube for extracting biological samples. Background technique [0002] Centrifugal technology has been widely used in biological sciences, especially in the fields of biochemistry and molecular biology research, and each biochemistry and molecular biology laboratory must prepare various types of centrifuges. Centrifugal technology is mainly used for the separation and preparation of various biological samples. The suspension of biological samples is placed in a centrifuge tube under high-speed rotation. Due to the huge centrifugal force, the suspended tiny particles (such as cell organelles, sedimentation of biological macromolecules, etc.) ) settles at a certain speed, thereby being separated from the solution, so the application range of the centrifuge tube is very wide. [0003] When storing or incubating b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J7/04C08J7/12C08L23/12
CPCC08J7/0427C08J7/12C08J2323/12C08J2483/04
Inventor 张衡丁贤明
Owner 赛宁(苏州)生物科技有限公司
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