Extraction method of L-hydroxyproline

Abstract

The invention provides an L-hydroxyproline extraction method, which comprises: (1) heating an L-hydroxyproline fermentation broth to a temperature of 40-50 DEG C, and filtering through a ceramic membrane to obtain a ceramic filtration liquid; (2) adding an adsorbent into the ceramic filtrate obtained in the step (1) for decoloration, and filtering out the adsorbent through a microfiltration membrane to obtain a decolorized solution; (3) cooling and crystallizing the decoloring solution obtained in the step (2); and (4) separating the crystals obtained in the step (3) from the mother liquor to obtain the L-hydroxyproline. The method disclosed by the invention is simpler in steps, can efficiently separate a finished product and improve the quality and the extraction rate, and is more environment-friendly due to less generated wastewater and solid waste.

Description

technical field [0001] The invention belongs to the technical field of amino acid extraction technology, and in particular relates to a method for extracting L-hydroxyproline from L-hydroxyproline fermentation broth. Background technique [0002] L-hydroxyproline is a common non-standard protein amino acid, and its content is an important indicator to measure the quality of collagen. Due to its special physical and chemical properties, it is widely used in the production of food additives, cosmetics and pharmaceutical intermediates. [0003] At present, there are mainly four methods for producing L-hydroxyproline at home and abroad: proteolytic extraction, chemical synthesis, microbial fermentation and enzymatic conversion. Among them, the proteolytic extraction method, chemical synthesis method and enzymatic conversion method generally have the disadvantages of high cost, low yield, and serious waste problems. Therefore, the production of L-hydroxyproline by microbial fer...

AI Technical Summary

Problems solved by technology

Among them, a large amount of acid-base wastewater will be generated in the process of adsorption and removal of anions and cations, the process route is complicated, and the cycle is too long, etc.

CN105777603A discloses a method for extracting L-hydroxyproline from L-hydroxyproline fermentation broth. First, the fermentation broth is micro-filtered, and then treated with anions and cations. This process will generate a large amount of waste water, and the process is complicated, It takes a long time; the ion exchange liquid is treated by nanofiltration membrane, and the power cost of this process is high; at the same time, the pH of the feed liquid needs to be adjusted many times during the extraction process, which can easily cause the inorganic salt of the material to exceed the standard, and generate a large amount of acid-base wastewater

This process route requires the use of sulfuric acid to adjust the pH, and the use of precipitants and chelating agents to treat the fermentation broth. The use of more raw and auxiliary materials will generate a large amount of solid waste; the mother liquor also needs to be treated with macroporous adsorption resin, and the process is relatively complicated.