In-vitro preservation method for sargassum fusiforme rhizoid germplasm resources

Abstract

The invention discloses an in-vitro preservation method for sargassum fusiforme germplasm resources, and mainly relates to a low-temperature and low-salinity preservation method taking sargassum fusiforme rhizoid as a germplasm preservation material. The method is characterized in that long-time in-vitro preservation of the sargassum fusiforme rhizoid under the conditions that the temperature is 8-10 DEG C and the salinity is 19-20%, and the growth rate and the germination rate of the sargassum fusiforme rhizoid in the preservation period are effectively inhibited. The preservation method is easy to operate, resources are saved, and survival and germination of the sargassum fusiforme rhizoid are not affected; besides, normal growth, germination and seedling formation of the sargassum fusiforme rhizoid can be recovered under the proper culture condition, so that the key problem that sargassum fusiforme indoor germplasm resources are difficult to preserve is effectively solved, the preservation time can reach 4 months or above, the rhizoid germination rate can reach 400% or above after recovery culture, and the seedling rate can reach 150% or above.

Description

technical field [0001] The invention relates to the technical field of aquaculture, in particular to a method for preserving germplasm resources in vitro by rhizomes of hijiki. Background technique [0002] Sargassum fusiforme is a large brown algae with high nutritional, medicinal, ecological and economic value. my country is one of the main countries in the world where hijiki is cultivated, and hijiki cultivation has important economic and ecological significance. [0003] In the entire life cycle of Hijiki, except for the breeding period of artificial sexual reproduction, which is carried out in nursery ponds, the rest of the life cycle is completed in sea areas, so there is a risk of loss of excellent germplasm resources under extreme climate conditions , such as typhoon loss and high temperature rot, etc.; there is also the risk of loss of traits of excellent lines due to genetic drift caused by sexual reproduction and breeding. Based on the above reasons, the long-te...

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Problems solved by technology

[0004] At present, the research on the preservation methods of indoor germplasm resources of Hijiki mainly includes ultra-low temperature and low-temperature preservation of fertilized eggs, low-temperature preservation of reproductive trays and stems, etc., but there is no successful case of long-term preservation. The bottleneck of this technology has not yet been broken through

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