Sample analyzer and counting method thereof

Abstract

The invention discloses a sample analyzer and a counting method thereof. The sample analyzer comprises a control module, a first detection module and a second detection module, wherein the control module is connected with the first detection module and the second detection module. The counting method comprises the following steps that the control module generates a first histogram based on first measurement data of the first detection module; the control module obtains an area ratio based on the ratio of the first area to the total area of the first histogram; the control module calculates the number ratio of the number of discrete particles to the number of white blood cells of the second detection module; the control module obtains a positive probability value of the sample based on a plurality of area ratios and the number ratio; and the control module obtains the proportion of basophilic granulocytes in the white blood cells based on the positive probability value. Through the mode, the positive misjudgment rate of the sample can be reduced, the positive detection rate of the sample can be increased, and counting value reliability of the basophilic granulocytes can be improved.

Description

technical field [0001] The present application relates to the technical field of sample analysis, in particular to a sample analyzer and a counting method thereof. Background technique [0002] Blood cell analyzer is an instrument used to classify and count blood cells. Usually, a blood cell analyzer can divide white blood cells into five groups: lymphocytes, monocytes, neutrophils, eosinophils, and basophils. It counts. [0003] The blood cell analyzer in the prior art measures the count of basophils in the sample through a single optical detection channel, wherein the basophils overlap with neutrophils and eosinophils in size and shape. In the absence of other abnormal particles in the basophil area of ​​the cell, the basophil count is incorrect. [0004] Alternatively, the hematology analyzer in the prior art measures the basophil count of the sample through a single impedance detection channel, which is easily affected by immature granulocytes, lipid particles, or chan...

AI Technical Summary

Problems solved by technology

[0003] The blood cell analyzer in the prior art measures the count of basophils in the sample through a single optical detection channel, wherein the basophils overlap with neutrophils and eosinophils in size and shape. Incorrect basophil counts in the presence of other abnormal particles in the basophil area of

[0004] Alternatively, the hematology analyzer in the prior art measures the basophil count of the sample through a single impedance detection channel, which is easily affected by immature granulocytes, lipid particles, or channel micropore clogging, resulting in basophils wrong count

[0005] Therefore, the blood cell analyzer in the prior art obtains the count value of basophils through a single channel, which has the problem of unreliability

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