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SSR marker, primer pair, application of SSR marker, and screening method of SSR marker sites related to early-maturing molecular breeding of upland cotton

A technology of molecular breeding and marking sites, which is applied in biochemical equipment and methods, measurement/testing of microorganisms, DNA/RNA fragments, etc., can solve the problems of unfavorable guidance-assisted selection, undiscovered, undetectable, etc., and achieve reliable molecular Effects of Marker Site Support

Active Publication Date: 2022-02-11
SHANDONG COTTON RES CENT
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Problems solved by technology

[0003] Cotton precociousness is an excellent comprehensive trait, mainly including traits such as full growth period, seedling stage, bud stage, flowering and boll stage, first fruiting branch position and pre-frost flowering rate, but these traits are quantitative traits and are affected by multiple quantitative traits. The control of trait gene loci, the genetic mechanism is complex and easily affected by environmental factors; traditional breeding methods mainly rely on the experience of breeders, and it is difficult to accurately grasp the traits related to precocity, so traditional breeding methods lack predictability and low efficiency
[0004] Cotton precociousness is a quantitative trait. Traditional genetic analysis methods are difficult to accurately track and locate the flow direction of the target gene in the hybrid offspring. The development of molecular marker technology provides an effective tool for the study of quantitative traits.
Linkage analysis and association analysis are the main methods to study the genotypes of plant quantitative traits at present. Using linkage analysis, the growth stage, seedling stage, bud stage, flowering and boll stage, first fruiting branch position, first fruiting branch height and pre-frost flowering have been located. QTL (Li C, Wang X, Na D, et al. QTL analysis for early-maturing traits in cotton using two upland cotton (Gossypium hirsutum L.) crosses[J]. Breeding Science, 2013, 63(2):154-163.), but the QTL mapping parent is not a production variety with a large promotion area in production, and the QTL mapping analysis cannot detect the alleles that exist but have no difference in the mapping population parents , and the located QTL has a strong "population specificity", so the research results of QTL mapping analysis based on linkage analysis are difficult to be directly applied to the genetic improvement of early maturity traits in cotton varieties
Linkage analysis has been used to obtain three SSR loci that are significantly associated with early maturity-related traits of upland cotton, among which CER0098-400 is significantly associated with the whole growth period, DPL0375-250 and HAU2414-147 are significantly associated with the initial node position of fruiting branches (Liang Bing et al. , Association Analysis of Upland Cotton Agronomic Traits and SSR (Simple Sequence Repeats) Markers, Acta Cotton Sinica, 26(5): 387-395), however, no molecular markers significantly associated with cotton precociousness-related traits obtained by using The loci guide detailed reports on cotton early-maturity breeding, because the early-maturity-related molecular marker loci of upland cotton obtained by association analysis have not been verified whether they are closely linked to cotton early-maturity target traits, and the target traits associated with these molecular markers are in early-maturity Breeding aspects not conducive to guiding assisted selection

Method used

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  • SSR marker, primer pair, application of SSR marker, and screening method of SSR marker sites related to early-maturing molecular breeding of upland cotton
  • SSR marker, primer pair, application of SSR marker, and screening method of SSR marker sites related to early-maturing molecular breeding of upland cotton
  • SSR marker, primer pair, application of SSR marker, and screening method of SSR marker sites related to early-maturing molecular breeding of upland cotton

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Embodiment 1

[0041] Embodiment 1 selects parents

[0042] Shandong Cotton Research Center used mid-to-late maturing conventional insect-resistant cotton variety Lumianyan No. 37 as the female parent, and insect-resistant short-season cotton Lumianyan No. 19 as the male parent to construct F2 and F4 populations respectively. According to the seedling bud stage (SSP, The number of days from sowing to cotton flowering) traits were used to identify and select the precocious individual plants of the F2 population and the F4 population. The parent, Lumianyan 37, is a new mid-late-maturing, insect-resistant cotton that was bred by crossing Lumianyan 16, a Bt gene-transferred conventional insect-resistant cotton line, Lu S6145, and the mid-early maturing elite line, Lu 9136, as the female parent. The variety has the characteristics of compact plant type, large leaves, tough stalks, and strong stress resistance; the parent Lumianyan 19 is the male parent of Lu 55, a conventional Bt gene-transferred...

Embodiment 2

[0044] (1) In 2014, one row of Lumianyan No. 37 and Lumianyan No. 19 were planted at the Linqing Experimental Station of Shandong Cotton Research Center. Lumianyan No. 37 was used as the female parent and Lumianyan No. 19 was used as the male parent to prepare a hybrid Combined to produce F1, mixed harvest after maturity, planted 2 rows of F1 in Sanya, Hainan in the winter of that year, self-crossed to obtain F2, F2 generation was harvested as a single plant, according to the traits of seedling bud stage (SSP, the number of days from sowing to cotton flowering), in About 1 / 8 of the early-maturing lines and about 1 / 8 of the late-maturing lines were selected from the F2 population, planted with F3, and self-crossed to obtain F4, and each generation was a mixed harvest. Field management was carried out by conventional methods.

[0045] (2) Construction of precocious directional selection population: In 2015, 20 rows of F2 were planted at the Linqing Experimental Station of Shando...

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Abstract

The invention discloses an SSR marker, a primer pair, application of the SSR marker, and a screening method of SSR marker sites related to early-maturing molecular breeding of upland cotton. The method comprises the following steps: by taking a mid-late-maturing cotton variety Lumianyan 37 as a female parent and an early-maturing cotton variety Lumianyan 19 as a male parent, hybridizing for one generation, then selfing for one generation to obtain F2, selecting an 1 / 8 early-maturing strain and an 1 / 8 late-maturing strain in the F2 group, planting F3, selfing to obtain F4, and carrying out first identification on the SSR marker sites related to the early-maturing characters of the cotton by utilizing the F2 group, performing chi-square analysis to preliminarily determine the characters in the seedling and bud stage, and selecting the effective SSR markers related to early-maturing molecular breeding of the upland cotton; and carrying out secondary identification of early-maturing related SSR marker sites by using the F4 group, performing chi-square analysis to finally determine the characters in the seedling and bud stage, and selecting effective upland cotton early-maturing molecular breeding related SSR markers. The molecular marker sites for early-maturing molecular breeding of upland cotton are obtained.

Description

[0001] This application is a divisional application with an application date of December 13, 2017, an application number of 201711328014.6, and an invention title of "A Method for Identification of SSR Marker Loci Related to Early Maturity Molecular Breeding of Upland Cotton". technical field [0002] The invention belongs to the technical field of crop breeding, and relates to SSR markers, primer pairs and applications thereof, and a screening method for SSR marker sites related to early-maturing molecular breeding of upland cotton. Background technique [0003] Cotton precociousness is an excellent comprehensive trait, mainly including traits such as full growth period, seedling stage, bud stage, flowering and boll stage, first fruiting branch position and pre-frost flowering rate, but these traits are quantitative traits and are affected by multiple quantitative traits. The control of genetic loci for traits has complex genetic mechanisms and is easily affected by environm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/6895C12Q1/686
CPCC12Q1/6895C12Q2600/156C12Q2600/13C12Q1/686C12Q2525/151
Inventor 刘国栋张军陈鹏云张传云王芙蓉周娟杜召海陈煜张景霞
Owner SHANDONG COTTON RES CENT
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