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Amelancheir alnifolia plant differential culture medium in test tube and culture method thereof

A differentiation medium and in-test tube technology, applied in botany equipment and methods, horticultural methods, plant regeneration, etc., can solve the problems of limited number of branches for cuttings, high cost, low survival rate, etc., and achieve rapid propagation of seedlings , Strong seed consistency and high survival rate

Pending Publication Date: 2022-02-11
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems of low survival rate, high cost and limited number of cutting branches in the existing Tangdi propagation method, the present invention provides a Tangdi test tube plant differentiation medium and its cultivation method

Method used

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  • Amelancheir alnifolia plant differential culture medium in test tube and culture method thereof
  • Amelancheir alnifolia plant differential culture medium in test tube and culture method thereof
  • Amelancheir alnifolia plant differential culture medium in test tube and culture method thereof

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specific Embodiment approach 1

[0017] Specific embodiment one: In this embodiment, the plant differentiation medium in the test tube is composed of macroelements, trace elements, hormones and agar, wherein, in each liter of differentiation medium, macroelements are 2445mg~2548mg, trace elements are 37~39mg, hormones 2.1mg~6mg, agar 6~8g; the macroelements in the medium are ammonium nitrate 900~920mg / L, potassium nitrate 1000~1050mg / L, potassium dihydrogen phosphate 90~100mg / L, magnesium sulfate 200~210mg / L, calcium chloride 240~250mg / L and disodium edetate 15~18mg / L; the trace elements in the culture medium are potassium iodide 0.8~0.9mg / L, boric acid 6~7mg / L, zinc sulfate 8~9mg / L, manganese sulfate 21~23mg / L, sodium molybdate 0.02~0.03mg / L, copper sulfate 0.2~0.3mg / L and cobalt chloride 0.02~0.03mg / L; the hormone in the culture medium is 6-benzylaminopurine 1~2mg / L (6-BA), indole butyric acid 0.5~1mg / L (IBA), gibberellin 0.5~2.0mg / L (GA3) and kinetin 0.1~1.0mg / L L(KT).

specific Embodiment approach 2

[0018] Specific embodiment two: the difference between this embodiment and specific embodiment one is: trace element is potassium iodide 0.83mg / L, boric acid 6.2mg / L, zinc sulfate 8.6mg / L, manganese sulfate 22.3mg / L, sodium molybdate 0.025 mg / L, copper sulfate 0.25mg / L, cobalt chloride 0.025mg / L.

specific Embodiment approach 3

[0019] Specific embodiment three: the method for cultivating Tang Di in test tube is carried out according to the following steps:

[0020] (1) Sample treatment: take Tangdi stems as explants, soak them in 75% alcohol for 10-15 seconds, rinse them with sterile water for 3-5 times, and then sterilize them with 0.1% mercury chloride solution for 6-10 minutes. Then rinse with sterile water for 3 to 5 times, and cut off both ends of the stem;

[0021] (2) The treated samples were inoculated onto the above-mentioned plant differentiation medium in Tangdi test tube for cultivation, subcultured once every 20-30 days, and the culture conditions were: culture temperature 24-26°C, light time 10-14h / d, Strength 1800~2000Lx.

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Abstract

The invention discloses an amelancheir alnifolia plant differential culture medium in a test tube and a culture method thereof, and relates to an amelancheir alnifolia plant differential culture medium and a culture method thereof. The amelancheir alnifolia propagation method solves the problems of low survival rate, high cost, and a limited number of branches for cuttage in a amelancheir alnifolia propagation method in the prior art. The culture medium consists of major elements, trace elements, hormones and agar. The method comprises the following steps: 1, sample treatment; 2, culture. According to the invention, the components in the culture medium promote cell differentiation and organ formation during culture, and the bud induction rate is increased, so that the problems that the amelancheir alnifolia propagation is difficult, and the number of branches for cuttage is limited are solved, the survival rate is high, and the cost is low.

Description

technical field [0001] The invention relates to a Tangdi plant differentiation medium and a cultivation method thereof. Background technique [0002] Amelancheir alnifolia (Amelancheir alnifolia) is a deciduous shrub or tree belonging to the family Rosaceae. It is 2-3m high. It germinates in early April in Harbin. The flowers and leaves are at the same time. The white flowers are clustered and self-pollinated. The flowering period is 20-25 days; the leaves are nearly round , the base of the leaf is nearly heart-shaped, the tip of the leaf is acuminate, and there are small serrations on the edge of the leaf; the branches are erect and reddish-brown; the fruit begins to change color in mid-to-early July and gradually matures, from green to red and then to purple-black, and the peel is covered with fruit powder , the fruit is spherical; the weight of a single fruit is 1.2-1.5g, 12-15 fruit grains are attached to a single ear, the weight of each ear is 15.5-18.5g, and the yield ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/002A01H4/005A01H4/008
Inventor 赵恒田冯雪婷臧丹丹刘淑华孙燕邵玲玲
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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