Improved cellulase combined complex microbial inoculant

Abstract

The invention discloses an improved cellulase combined complex microbial inoculant. The improved cellulase combined complex microbial inoculant is prepared from the following components in parts by mass: 0.01 to 65 parts of leuconostoc mesenteroides, 0.05 to 15 parts of lactobacillus plantarum, 0.01 to 8 parts of lactobacillus buchneri, 0.01 to 87 parts of cellulase and 0.01 to 77 parts of xylanase. An application method of the improved cellulase combined complex microbial inoculant comprises the following steps: carrying out genetic engineering breeding; injecting mutated cellulase gene plasmids into the most common enzyme-producing engineering bacterium Trichoderma reesei by virtue of a genetic recombination technology; and directly combining the mutated plasmids with a template in a Trichoderma reesei division process. According to biochemical experiment determination of the improved cellulase combined complex microbial inoculant, the enzyme activity of mutated improved enzyme can be increased by about 10%, the application of glucosidase can be reduced in industrial production, and the higher enzyme activity of the improved cellulase combined complex microbial inoculant is very suitable for the actual demand of low-temperature fermentation in winter when the improved cellulase combined complex microbial inoculant is used for agricultural straw degradation and fertilizer preparation.

Description

technical field [0001] The invention relates to the technical field of composite bacterial agents, in particular to an improved cellulase combined with composite bacterial agents. Background technique [0002] At present, there are two main ways to use straw. One is to collect it centrally for power generation, heating or to make biofuel. Not many are used. The second is returning straw to the field, but direct returning of straw to the field has many disadvantages, including slow degradation, high carbon-to-nitrogen ratio, supplementary nitrogen fertilizer, high crushing and plowing process requirements, etc. Moreover, there is an upper limit on the weight of direct return to the field, generally no more than 200 kilograms per mu of land, and the excess part still cannot be handled. [0003] Therefore, a good way to return to the field is to decompose the straw first, degrade the cellulose in it, and then return to the field. However, the temperature in the winter slack p...

AI Technical Summary

Problems solved by technology

[0002] At present, there are two main ways to use straw. One is to collect it centrally for power generation, heating or to make biofuel. not used much

The second is to return the straw to the field, but the direct return of the straw to the field has many disadvantages, including slow degradation, high carbon-to-nitrogen ratio, supplementary nitrogen fertilizer, and high requirements for crushing and plowing techniques, etc.

Moreover, there is an upper limit on the weight of direct return to the field, generally no more than 200 kilograms per mu of land, and the excess part still cannot be handled

[0003] Therefore, a good way to return to the field is to decompose the straw first, degrade the cellulose in it, and then return to the field. However, the temperature in the winter slack period in northern my country is very low. At low temperature, the heat generation rate is not as fast as the heat dissipation rate, which will lead to problems such as incomplete corrosion