Multi-LED multiplexing 3D-FPM reconstruction algorithm based on multilayer diffraction model

Abstract

A multi-LED multiplexing 3D-FPM reconstruction algorithm based on a multi-layer diffraction model relates to the technical field of computational imaging, solves the problem that the acquisition rate is too slow when a plurality of LEDs illuminate, and comprises the following steps: step 1, constructing a loss function between a low-resolution picture obtained by the multi-layer diffraction model and a low-resolution picture actually acquired by a camera; step 2, updating a sample refractive index function of the thin sample layer by adopting a gradient descent method by utilizing the newest loss function, the newest incident light complex amplitude of the thin sample layer and the newest sample refractive index function of the thin sample layer, and updating a phase part of a newest pupil function by utilizing the gradient descent method; and step 3, judging whether the number of times of completing the step 2 reaches a preset number, if so, outputting a sample refractive index function, and otherwise, performing the step 1 again according to an updated result. According to the method, the number of LR pictures needing to be collected is greatly reduced, meanwhile, the influence of system aberration on a reconstruction result is effectively reduced, and the reconstruction precision of an algorithm is improved.

Description

technical field [0001] The invention relates to the technical field of computational imaging, in particular to a multi-LED multiplexing 3D-FPM reconstruction algorithm based on a multi-layer diffraction model. Background technique [0002] Fourier Stack Microscopy (FPM) is a newly developed imaging method designed to circumvent the limitation of the spatial bandwidth product (SBP) and obtain wide-field, high-resolution complex images. Since 2013, Fourier stack imaging technology has been applied in optical microscopy, biomedicine, life science and other fields to obtain microscopic images with large field of view and high resolution. At the same time, there has been some progress in the 3D reconstruction of thick samples, but for 3D reconstruction, the number of LEDs in the light source has to be greatly increased, which will lead to a long acquisition time of the system. For the observation of living samples such as biological cells cultured in vitro Not applicable, curren...

AI Technical Summary

Problems solved by technology

[0003] In order to solve the problem that the acquisition rate is too slow due to the large number of light source LEDs in Fourier stack imaging in 3-dimensional reconstruction, the present invention proposes a multi-LED multiplexing coding lighting strategy (that is, lighting multiple LEDs at the same time for lighting) , reducing the number of pictures required while ensuring the same amount of collected information) A multi-LED multiplexing 3D-FPM reconstruction algorithm based on the multi-layer diffraction model was built

Images