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Method for promoting growth and disease resistance of vegetables by using paenibacillus polymyxa AF01

A polymyx spore-like and vegetable technology, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, and botanical equipment and methods, can solve problems such as in-depth research on the cultivation of bacterial strains, and achieve good economic effects and strong flavor. , the effect of improving the quality of cucumber

Active Publication Date: 2022-01-14
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The applicant's research group has been committed to the research of biocontrol bacteria for a long time, and applied for a patent application entitled "A Paenibacillus polymyxa biocontrol strain AF01 and its application" on November 30, 2017 with the application number 201711244499.0. Published the Paenibacillus polymyxa AF01 screened by the applicant and its application, which proved that the strain AF01 can inhibit a variety of pathogenic bacteria, but the article did not conduct in-depth research on the cultivation of the strain. After research, it was found that in addition to the bacteriostatic effect disclosed in the patent 201711244499.0, it also has a good effect of inhibiting other pathogens and promoting crop growth. Because biocontrol bacteria have different effects on different vegetable crops, they have inhibitory effects on certain pathogenic bacteria. Some pathogenic bacteria do not necessarily have an inhibitory effect, and the growth-promoting effect on some plants does not necessarily have a growth-promoting effect on other plants, and these effects are unpredictable, and researchers need to continue to verify through experiments. The new application of the strain still needs continuous research

Method used

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  • Method for promoting growth and disease resistance of vegetables by using paenibacillus polymyxa AF01

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Embodiment 1

[0027] What this embodiment studies is the inhibitory effect of Paenibacillus polymyxa AF01 on different vegetable pathogens, and the specific methods are as follows:

[0028] Adopt confrontation growth method to measure the inhibitory effect of antagonistic bacterial strain AF01 of the present invention to different pathogens: get 5mm different pathogens (pathogens are: tomato black spot bacterium (Alternaria alternata), tomato anthracnose bacterium (Colletotrichum gloeosporioides), cucumber target spot Bacteria (Corynespora cassiicola), cucumber wilt (Fusarium oxysporum)) bacteria cakes are placed in the center of the PDA plate;

[0029] Point 1 μL with a pipette gun at a distance of 2.5 cm from the bacteria cake to a concentration of 10 9 Cfu / mL AF01 culture solution was cultured in a 28°C incubator, and the inhibition zone or zone was observed.

[0030] The experimental results are shown in Table 1 and Figure 1-4

[0031] Wherein, as can be seen from the results in Tab...

Embodiment 2

[0037] What this embodiment studies is the growth-promoting effect of Paenibacillus polymyxa AF01 on cucumber:

[0038] Inoculate Paenibacillus polymyxa AF01 to xylose 1.4%, NH 4 h 2 PO 4 1.0%, CaCl 2 0.6%. In a liquid medium with an initial pH value of 5, cultivate for 48 hours, dilute the fermentation broth by 10 times and 20 times, that is, the number of viable bacteria is 1.2×10 8 and 6×10 7 cfu / mL. Sow cucumbers into seedling trays containing seedling substrates, sow 1 seed in each hole, and spray evenly with bacterial solution on the trays at the time of sowing and 7 days later, 20ml in each hole. There were 30 holes for each treatment, and the blank control was gonorrhea sterile medium. After 20 days, observe the growth of the root system of the plant, and the effect of promoting growth can be seen Figure 5 .

[0039] Figure 5 Figure ① in Figure 1 is the experimental result of the 10-fold dilution of the fermentation broth, Figure ② is the experimental res...

Embodiment 3

[0041] What this embodiment studies is the application of Paenibacillus polymyxa AF01 in cucumber cultivation:

[0042] The test site of this embodiment: in Linlu Village, Wuming District, Nanning City;

[0043] Cucumber variety: Guiqing No. 1;

[0044] The planting application method is as follows:

[0045] Treatment 1: use PDA medium to prepare seed solution, and inoculate the seed solution containing 1.4% xylose, NH 4 h 2 PO 4 1.0%, CaCl 2 0.6%. In a liquid medium with an initial pH value of 5, shake culture for 48-72 hours to obtain a fermentation broth of AF01. When using, dilute the fermentation broth 20 times, that is, the number of viable bacteria is 2-6×10 7 cfu / mL. When the cucumber is transplanted, apply 0.5 kg of organic fertilizer to the hole first, and then use the diluted fermented liquid to irrigate the roots after transplanting, 200 ml per plant. Dilute 40 times at intervals of 10-14 days, that is, the number of viable bacteria is 1-3×10 7 cfu / mL, ...

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Abstract

The invention relates to the technical field of biology, in particular to a method for promoting growth and disease resistance of vegetables by using paenibacillus polymyxa AF01. The invention relates to novel application of the strain. Experiments prove that the strain AF01 has an inhibiting effect on various vegetable disease pathogenic bacteria such as tomato alternaria alternata, tomato colletotrichum gloeosporioides, cucumber target leaf spot bacteria and cucumber fusarium wilt bacteria; meanwhile, the strain can well promote growth of cucumber roots, increase the yield of cucumbers, remarkably improve the content of nutrient substances such as vitamin C, protein, soluble solids and total phenols of cucumbers and improve the flavor and taste of cucumbers; meanwhile, the strain AF01 disclosed by the invention also has a certain prevention effect on the powdery mildew of the bitter gourds, is an excellent biocontrol strain which is rare and has the effects of inhibiting bacteria, promoting the growth of the cucumbers, improving the quality of the cucumbers and inhibiting the occurrence of the powdery mildew of the bitter gourds, and has a good economic effect on cucumber planting.

Description

【Technical field】 [0001] The invention relates to the field of biotechnology, in particular to a method for promoting vegetable growth and disease resistance by using Paenibacillus polymyxa AF01. 【Background technique】 [0002] Solanaceous vegetables such as tomato and melon vegetables such as cucumber and bitter gourd have the advantages of high yield, long growth and supply season, and wide range of economic utilization. They are widely planted vegetable varieties in my country, but there are anthracnose, powdery mildew and The widespread occurrence of diseases such as Fusarium wilt and the abuse of pesticides and fertilizers hinder the healthy and green development of tomato and melon vegetables. Plant growth promoting rhizobacteria, including Paenibacillus polymyxa, is a general term for beneficial bacteria that live in the soil or rhizosphere and can promote plant growth or inhibit pathogenic bacteria in plants. Paenibacillus polymyxa can directly promote the growth-pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01G22/05A01G13/00A01N63/22A01P3/00C12R1/12
CPCC12N1/20A01G22/05A01G13/00A01N63/22
Inventor 林珊宇覃丽萍谢玲龙艳艳张艳曾凤花
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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