Probe method for detecting human IDH1 gene R132H mutation and kit thereof

Abstract

The invention discloses a probe method for detecting human IDH1 gene R132H mutation and a kit thereof, and relates to the technical field of biological medical treatment. The probe method for detecting the human IDH1 gene R132H mutation comprises the following steps of: (1) adding human genome DNA into an IDH1R132HPCR reaction mixed solution to form an R132H reaction system, and performing fluorescent quantitative PCR amplification; (2) result judgment: performing analysis according to a mutation Ct value of PCR amplification, wherein the IDH1R132H reaction mixed solution comprises an IDH1R132H forward primer with a sequence shown in SEQ ID No. 3 in a sequence table, an IDH1R132H reverse primer with a sequence shown in SEQ ID No. 4 in the sequence table and a PCR premix solution containing a fluorescent dye. The probe method and the kit have the beneficial effects that the probe method and the kit provide high-sensitivity detection of the IDH1 gene R132H mutation for clinical application, and the IDH1 gene R132H mutation detection is high in speed, high in efficiency and high in specificity.

Description

technical field [0001] The invention relates to the field of biomedical technology, in particular to a probe method and a kit for detecting the R132H mutation of human IDH1 gene. Background technique [0002] The isocitrate dehydrogenase (IDH) family includes enzymes that use NAD or NADP as cofactors to catalyze the oxidative decarboxylation of isocitrate to generate α-ketoglutarate, and simultaneously generate NADH or NADPH, respectively. In mammalian cells, IDH isozymes have the following three forms: NAD-dependent mitochondrial IDH1, NADP-dependent mitochondrial IDH2, and NADP-dependent cytosolic IDH3. The IDH1 gene encoding the NAD-dependent human IDH1 is located on chromosome 2q33.3 and localized in the cytoplasm and peroxisomes; the IDH2 gene encoding the NADP-dependent mitochondrial IDH2 enzyme is located on chromosome 15q26.1, but the human IDH1 gene R132H is now detected The sensitivity detection rate of the mutant probe method is relatively low, and the speed is s...

AI Technical Summary

Problems solved by technology

The IDH1 gene encoding the NAD-dependent human IDH1 is located on chromosome 2q33.3 and localized in the cytoplasm and peroxisomes; the IDH2 gene encoding the NADP-dependent mitochondrial IDH2 enzyme is located on chromosome 15q26.1, but the human IDH1 gene R132H is now detected The sensitivity detection rate of the mutation probe method is relatively low, and the speed is slow and its specificity is not strong