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Application of five biomarkers in preparation or screening of liver cancer diagnostic reagent

A biomarker, cancer diagnosis technology, applied in the field of five biomarkers in the preparation or screening of diagnostic reagents for liver cancer, can solve problems such as tumor recurrence, and achieve the effect of predicting the risk of cancer recurrence

Pending Publication Date: 2021-12-28
深圳市龙华区中心医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in these patients, tumor recurrence is often a lethal factor threatening survival

Method used

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  • Application of five biomarkers in preparation or screening of liver cancer diagnostic reagent
  • Application of five biomarkers in preparation or screening of liver cancer diagnostic reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0118] The kit contains:

[0119] Trizol lysate (Invitrogen, 15596-026);

[0120] RNAse-free homogenizer;

[0121] RNAse-free 1.5ml tube;

[0122] Chloroform;

[0123] isopropanol;

[0124] Washing solution (75% V / V ethanol prepared with RNAse-free water);

[0125] RNAse-free water;

[0126] Reverse transcription reagent mixture (10 μL / tube, TAKARA, RR047A);

[0127] Q-PCR reagent mix (TAKARA, 639676);

[0128] Primer diluent (aqueous solution containing primers).

[0129] The operation steps are as follows:

[0130] 1. Tumor sample preparation: For patients with multiple liver cancer foci, a piece of tumor (50 mg / part) was taken from each foci, and a total of 3 foci were taken; for patients with less than 3 liver cancer foci, random Take 3 tumor blocks (50 mg / part); the tumor tissues are labeled as HCC1, HCC2, HCC3. Take 3 pieces of paracancerous tissues randomly (50 mg / part), and mark Nor1, Nor2, Nor3.

[0131] 2. Cut the tissue as small as possible with sterilize...

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Abstract

The invention discloses application of five biomarkers in preparation or screening of a liver cancer diagnostic reagent. The biomarkers comprise at least one of following genes: CD47, Tcf19, Cldn3, Sox4 and Sox9. The five biomarkers can be used for effectively predicting the risk of cancer recurrence of a patient and provide assistance for clinical treatment.

Description

technical field [0001] The invention relates to the medical field, in particular to the use of five biomarkers in the preparation or screening of liver cancer diagnostic reagents. Background technique [0002] Cancer is one of the major diseases that threaten human health, and also shortens human life span. For example, liver cancer can cause damage to liver function. Among them, hepatocellular carcinoma (HCC) is one of the top five cancer killers in the world. Hepatectomy is the most commonly used treatment, achieving a 70% 5-year survival rate. However, in these patients, tumor recurrence is often a lethal factor threatening survival. Existing studies have shown that nearly 70% of patients will relapse within 5 years after surgery. Contents of the invention [0003] According to the first aspect, in one embodiment, the use of biomarkers in the preparation or screening of cancer diagnostic reagents is provided, the biomarkers include at least one of the following genes:...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886G16B25/10G16B25/20G16H50/30
CPCC12Q1/6886G16B25/10G16B25/20G16H50/30C12Q2600/118C12Q2600/136C12Q2600/158C12Q2600/166
Inventor 赵力敏李雅婧陈协辉陈鹏飞
Owner 深圳市龙华区中心医院
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