Liquid chromatography-DAD-tandem mass spectrometry method for simultaneously detecting four enantiomers in cyfluthrin and application of liquid chromatography-DAD-tandem mass spectrometry method

Abstract

The invention provides a liquid chromatography-DAD-tandem mass spectrometry method for simultaneously detecting four enantiomers in cyfluthrin and application of the liquid chromatography-DAD-tandem mass spectrometry method, and the method comprises the following steps: dissolving a sample to be detected in chromatographic grade absolute ethyl alcohol to obtain a detection solution, and detecting the detection solution by adopting a liquid chromatography-DAD-tandem mass spectrometry technology, wherein the liquid chromatography adopts reversed-phase liquid chromatography, the mobile phase is a water-free alcohol solution, and the mass spectrum adopts a DAD-ultraviolet detector. According to the method, absolute ethyl alcohol is selected as the mobile phase, so that the maximum separation effect of four enantiomers can be ensured, and the requirement that a chiral chromatographic column cannot use any water phase system can be met; 0.2-0.45 v% of ammonia water is added into the mobile phase, so that the cyfluthrin is ionized, and the detection sensitivity is improved; and according to the method, a DAD-ultraviolet detector is matched, so that the mass spectrum response of the method is improved, parameters such as collision energy are optimized, and quantitative analysis of four enantiomers of the cyfluthrin is realized.

Description

technical field [0001] The invention relates to the technical field of analysis and detection, more specifically, to a liquid chromatography-DAD-tandem mass spectrometry method for simultaneously detecting four enantiomers in cyfluthrin and its application. Background technique [0002] So far, more than 25% of the compounds in agricultural production have chiral centers; most of them are produced, sold, applied and discharged into the environment as racemates. Therefore, the environmental safety of chiral pesticides is a research hotspot in current environmental science, which needs to be studied and improved at the enantiomeric level. less. [0003] Cyfluthrin strips are widely used in many countries including my country as a drug against mites. Its trade name is Bayvarol, and its Chinese trade name is Fengmite Qing. It is produced by Bayer AG of Germany and has been around since the 1990s. It is registered and used in my country; however, due to the high price, its promo...

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Problems solved by technology

[0007] Cyfluthrin has certain residual hidden dangers in the hive system and has a certain risk of toxicity to bees, and the structure similarity of its enantiomers is too high, which leads to enantiomers in p-cyfluthrin Detection of isomers is challenging

[0008] Up to now, research on the environmental behavior of cyfluthrin at the level of enantiomers has not yet been carried out, and no qualitative and quantitative studies on the enantiomers in cyfluthrin have been seen. detection

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