Simultaneous detection of four enantiomers in flufluthrin by liquid chromatography-dad-tandem mass spectrometry and its application

Abstract

The invention provides a liquid chromatography-DAD-tandem mass spectrometry method for simultaneously detecting 4 enantiomers in cyfluthrin and its application. The method comprises dissolving the sample to be tested in chromatographic grade anhydrous Obtain detection solution in ethanol, adopt liquid chromatography-DAD-tandem mass spectrometry technology to detect detection solution; ‑UV detector. In the present invention, by selecting absolute ethanol as the mobile phase, the maximum separation effect of the four enantiomers and the requirement that any aqueous phase system cannot be used for the chiral chromatographic column can be guaranteed; by adding 0.2-0.45 v% to the mobile phase The ammonia water ionizes cyfluthrin and improves the detection sensitivity; by using a DAD-UV detector, its mass spectrometry response is improved, and parameters such as collision energy are optimized, and 4 cyfluthrin Quantitative analysis of enantiomers.

Description

technical field [0001] The invention relates to the technical field of analysis and detection, more specifically, to a liquid chromatography-DAD-tandem mass spectrometry method for simultaneously detecting four enantiomers in cyfluthrin and its application. Background technique [0002] So far, more than 25% of the compounds in agricultural production have chiral centers; most of them are produced, sold, applied and discharged into the environment as racemates. Therefore, the environmental safety of chiral pesticides is a research hotspot in current environmental science, which needs to be studied and improved at the enantiomeric level. less. [0003] Cyfluthrin strips are widely used in many countries including my country as a drug against mites. Its trade name is Bayvarol, and its Chinese trade name is Fengmite Qing. It is produced by Bayer AG of Germany and has been around since the 1990s. It is registered and used in my country; however, due to the high price, its promo...

AI Technical Summary

Problems solved by technology

[0007] Cyfluthrin has certain residual hidden dangers in the hive system and has a certain risk of toxicity to bees, and the structure similarity of its enantiomers is too high, which leads to enantiomers in p-cyfluthrin Detection of isomers is challenging

[0008] Up to now, research on the environmental behavior of cyfluthrin at the level of enantiomers has not yet been carried out, and no qualitative and quantitative studies on the enantiomers in cyfluthrin have been seen. detection