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An insecticidal essential oil of Artemisia annua and its application

A technology of wormwood and essential oil, applied in the field of botanical pesticides, can solve the problems of unreported insecticidal activity, and achieve the effect of easy-to-obtain, simple reagents and strong drug properties

Active Publication Date: 2022-08-05
INST OF PLANT PROTECTION NINGXIA ACAD OF AGRI & FORESTRY SCI KEY LAB OF NINGXIA PLANT DISEASE & INSECT PESTS CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The essential oil of Artemisia chinensis has been disclosed to have repellent activity against tobacco beetles and book lice, but there is no information about the insecticidal activity of Artemisia spp. to report

Method used

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  • An insecticidal essential oil of Artemisia annua and its application
  • An insecticidal essential oil of Artemisia annua and its application
  • An insecticidal essential oil of Artemisia annua and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] This example provides the preparation process and GC-MS test of Artemisia annua essential oil.

[0016] The aerial parts of Artemisia chinensis plants were naturally dried in the shade, pulverized, weighed 500g and placed in an essential oil extractor, extracted by steam distillation, and the distillation time was 6h. The distillate was extracted with n-hexane to obtain a n-hexane layer, anhydrous sodium sulfate was added to remove water, after concentration, the volume was measured, the essential oil was put into a dry glass bottle, and placed in a 4°C refrigerator for storage, that is, the North China Rice Artemisia essential oil.

[0017] The essential oil samples of Artemisia annua were analyzed by GC-MS. The test conditions were: GCMS-QP2010plus (Shimadzu); chromatographic column: DB-5MS (60m×0.25μm×0.32mm) (Agilent); heating rate: 60°C -10℃ / min-300℃-20min; split ratio: 30:1; constant linear velocity mode: 30cm / sec; carrier gas: He; ion source temperature: 200℃; i...

Embodiment 2

[0027] This example provides the determination of the contact killing activity of Artemisia annua essential oil on cotton aphid and psyllid.

[0028] (1) Determination of contact killing activity of Artemisia annua essential oil on cotton aphid

[0029] The insecticidal activity was determined by the leaf disc method. Polytetrafluoroethylene was applied to the four walls of a 12-well plate. After drying, the agar was spread on the bottom of the plate. Fresh cotton leaves without pesticides were taken, and the leaves were punched to make a A circular leaf disc with a diameter of 3cm, the leaves are soaked in medicine for 10s, then air-dried and placed in the orifice plate, inserted with 10 wolfberry cotton aphids, and placed in a light / dark=16h / 8h, 25℃, humidity of 60%~70% in the incubator. Negative and positive controls were used without the addition of Artemisia annua essential oil solvent and pyrethrin. Each treatment was repeated 6 times, with 10 cotton aphids per repetit...

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Abstract

The invention discloses an insecticidal Artemisia chinensis essential oil. The above-ground parts of the plants of Artemisia chinensis are naturally dried in the shade, pulverized, weighed, placed in an essential oil extractor, and extracted by steam distillation. 6h, extracted with n-hexane to obtain n-hexane layer, which was prepared by adding anhydrous sodium sulfate to remove water. The invention also provides the application of the essential oil of Artemisia annua in insecticidal, and the essential oil of Artemisia annua can effectively achieve contact killing of cotton aphids and psyllids.

Description

technical field [0001] The invention belongs to the technical field of botanical pesticides, relates to a Compositae, in particular to an insecticidal Artemisia annua essential oil and applications thereof. Background technique [0002] Psyllids and cotton aphid are common pests on citrus. These pests usually gather on the branches, leaves or fruits of citrus and harm by sucking sap, causing citrus branches withered, fallen leaves and fruit drop, and can produce secretions and induce soot sick. Among them, citrus psyllid is the only natural transmission vector of citrus Huanglongbing which is the most difficult and most threatening in citrus production. At present, the control of these pests is still dominated by chemical pesticides. However, the frequent use of chemical pesticides brings about increasingly serious problems such as drug resistance, environmental pollution and pesticide residues. With people's attention to the ecological environment, food safety and other i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/12A01P7/04
CPCA01N65/00A01N65/12
Inventor 刘畅黄文广王芳张蓉何嘉魏淑花王颖姜彩鸽宋双朱猛蒙
Owner INST OF PLANT PROTECTION NINGXIA ACAD OF AGRI & FORESTRY SCI KEY LAB OF NINGXIA PLANT DISEASE & INSECT PESTS CONTROL
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