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Method for culturing umbilical cord tissue mesenchymal cells

A technology of umbilical cord tissue and cells, applied in bone/connective tissue cells, tissue culture, animal cells, etc., can solve the problems that umbilical cord mesenchymal stem cells are rarely studied and reported, and achieve short cell growth cycle and fast growth rate , the effect of clear ingredients

Pending Publication Date: 2021-12-03
天晴干细胞股份有限公司
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  • Application Information

AI Technical Summary

Problems solved by technology

Since the umbilical cord is neonatal waste and there are no ethical issues, the acquisition of umbilical cord mesenchymal stem cells has been increasingly valued and studied. By adding exosomes to culture umbilical cord tissue Wharton's glue, a large number of umbilical cord mesenchymal stem cells can be quickly isolated. Mesenchymal stem cells are rarely studied and reported

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  • Method for culturing umbilical cord tissue mesenchymal cells
  • Method for culturing umbilical cord tissue mesenchymal cells
  • Method for culturing umbilical cord tissue mesenchymal cells

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Embodiment 1

[0030] A method for culturing umbilical cord tissue mesenchymal cells using exosomes in this embodiment is carried out according to the following steps:

[0031] 1. Isolation of umbilical cord tissue mesenchymal cells:

[0032] Collect the umbilical cord tissue that is qualified for virus detection, and complete the separation and preparation within 24 hours. In a sterile workbench, disinfect the umbilical cord with alcohol with a concentration of 75% by volume, then wash it twice with normal saline, cut the cleaned umbilical cord into 2-3 cm lengths, and place them in a dish. Peel off the Huatong glue, put the Huatong glue in a clean centrifuge tube, and cut it to 1-2mm with surgical scissors 3 block;

[0033] 2. Preparation of exosomes:

[0034] Select the umbilical cord mesenchymal stem cells of the P3-P5 generation, culture them in serum-free medium for 48-72 hours, collect 30-40 mL of the culture supernatant, and centrifuge at 1 000-1300 × g for 10-15 min to remove cel...

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Abstract

The invention discloses a method for culturing umbilical cord tissue mesenchymal cells, and relates to the field of cell culture. The invention provides the method for culturing umbilical cord tissue mesenchymal cells by adopting exosomes. According to the method, Wharton-type jelly is obtained from the umbilical cord tissue, then the exosome is obtained from the umbilical cord mesenchymal stem cells, and the obtained exosome is added into the culture medium to culture the Wharton-type jelly, so that the culture period is shortened, and the number of cells is increased within a short time. The method is applied to the field of umbilical cord tissue mesenchymal cell culture.

Description

technical field [0001] The invention relates to the field of cell culture, in particular to a method for culturing umbilical cord tissue mesenchymal cells. Background technique [0002] The umbilical cord is the link between the newborn and the mother. The tissue discarded after the birth of the newborn contains a large amount of Wharton's glue in the umbilical cord, and the Wharton's glue contains a large amount of umbilical cord mesenchymal stem cells. An important member of the stem cell family in biology, it is derived from the mesoderm and ectoderm of embryos in the early stage of development. It was originally found in the bone marrow of the population. The main clinical features are self-renewal, multi-directional differentiation, hematopoietic support, immune Its clinical use has attracted more and more attention of the population. Under certain induction conditions in vivo or in vitro, it can be differentiated into a variety of human tissue cells such as fat, muscl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
CPCC12N5/0668C12N2501/91C12N2501/90C12N2502/1388
Inventor 王灵娟张怡蔡光辉王博昊刘艳青
Owner 天晴干细胞股份有限公司
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