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Application and composition for preeclampsia diagnosis based on microRNA

A diagnostic composition and a technology for preeclampsia are applied in the fields of biomedicine and molecular biology to achieve the effect of easy dynamic monitoring

Pending Publication Date: 2021-11-30
THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports on microRNA in peripheral blood for predicting preeclampsia

Method used

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  • Application and composition for preeclampsia diagnosis based on microRNA
  • Application and composition for preeclampsia diagnosis based on microRNA
  • Application and composition for preeclampsia diagnosis based on microRNA

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1 research object

[0054] In this study, from January 2019 to June 2021, a total of 200 cases of medical records were collected by the prenatal diagnosis laboratory, divided into preeclampsia (PE) group and normal control group, and the case data, placental tissue samples, venous blood.

Embodiment 2

[0055] The collection of embodiment 2 placental tissue samples

[0056] Immediately after the placenta was delivered by cesarean section, two pieces of placenta with a size of about 1cm×1cm×1cm and a little decidua tissue were taken from the center of the mother’s surface of the placenta under aseptic condition, and the specimens were placed in a sterile Eppendorf tube and placed in liquid nitrogen for 15 minutes. Save until RNA extraction. Real-time PCR was used to detect the miR-486-5p level in the placenta tissue of each group.

Embodiment 3

[0057] The collection of embodiment 3 venous blood

[0058] Pregnant women draw 5ml of venous blood before giving birth and collect it in EDTA vacuum tube. The extracted venous blood was centrifuged in a centrifuge at 4°C and 1500g for 15 minutes within 30 minutes to separate the plasma and the buffy coat for RNA extraction.

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Abstract

The invention provides a method for diagnosing whether a subject has preeclampsia or is at the risk of preeclampsia, and further provides application of microRNA and / or a specific probe / detection primer of the microRNA in preparation of a diagnostic composition and the diagnostic composition for detecting preeclampsia. Expression of the microRNA in the plasma in a preeclampsia patient is obviously increased, and the microRNA can be used as a diagnostic index which is valuable, simple, convenient and easy to dynamically monitor preeclampsia; meanwhile, the increase of the microRNA in a placenta tissue is also a potential diagnostic index for predicting preeclampsia.

Description

technical field [0001] The invention belongs to the technical fields of biomedicine and molecular biology, and specifically relates to the application of peripheral blood microRNA 486 in the diagnosis and treatment of preeclampsia. Background technique [0002] Disclosing the information in this background technology section in the current market is only intended to increase the understanding of the general background of the present invention, and is not necessarily regarded as an acknowledgment or in any form to imply that the information constitutes existing knowledge that has become known to those skilled in the art. have technology. [0003] Preeclampsia (PE) is an idiopathic disease of pregnancy with unknown etiology. It is currently estimated to affect 2-8% of pregnant women worldwide and is one of the leading causes of maternal and perinatal death worldwide. one. According to the "Guidelines for the Diagnosis and Treatment of Hypertensive Disorders During Pregnancy ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12N15/11C12N15/113
CPCC12Q1/6883C12N15/113C12Q2600/158C12Q2600/178C12N2310/141
Inventor 刘世国张璐王敬丽张肖秦仕婷
Owner THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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