Bimodal microscopic imaging system and imaging method thereof

Abstract

The invention discloses a bimodal microscopic imaging system and an imaging method thereof. According to the bimodal microscopic imaging system combining two-photon fluorescence and optical diffraction tomography, synchronous control is achieved through the control subsystem, and the problems encountered by two-photon fluorescence imaging are solved by means of the characteristics of no mark, no invasion and small phototoxicity of optical diffraction tomography; meanwhile, a result in diffraction chromatography is calibrated by utilizing two-photon fluorescence, so that a biological sample is imaged from morphological and chemical specificity, and the two-photon fluorescence imaging device is simple and can realize relatively high resolution without a complicated imaging light path; the two modalities are fused based on the control subsystem, and parallel imaging characterization of local specificity and global morphology of the sample is achieved; and according to the invention, the three-dimensional high-resolution refractive index distribution of the to-be-detected sample can be recovered through optical diffraction tomography, and co-positioning is carried out on the two-photon fluorescence imaging of the to-be-detected sample at the same time, so that bimodal microscopic imaging is realized.

Description

technical field [0001] The invention relates to the field of micro-imaging technology, in particular to a dual-mode micro-imaging system combined with two-photon fluorescence and optical diffraction tomography and an imaging method thereof. Background technique [0002] Optical microscopy is an important invention in human history. After four centuries of history, the field of microscopic imaging has been continuously expanding, and different methods have emerged in an endless stream. Microscopic imaging technology uses the interaction between light and matter to magnify tiny objects that are difficult to observe, thereby presenting a microscopic scene that can be distinguished by the human eye. Especially in the field of biomedicine, optical microscopy technology, as an indispensable means of observation, reveals the process of life and promotes human exploration of life science. In order to be able to explain the life process in the microscopic field, the imaging capabili...

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Problems solved by technology

[0006] In order to solve the above existing problems in the prior art, the present invention proposes a dual-mode microscopic imaging system and its imaging method combining two-photon fluorescence and optical diffraction tomography. Through the synchronous control of the control subsystem, the optical diffraction layer is used to The characteristics of label-free, non-invasive, and low phototoxicity of analytical technology solve the problems encountered in two-photon fluorescence imaging; at the same time, two-photon fluorescence is used to calibrate the results of diffraction chromatography, so as to analyze biological samples in terms of morphology and chemical specificity. Imaging, and the two-photon fluorescence imaging device is simple and can achieve high resolution without complex imaging optical paths; based on the control subsystem, the two modes are fused to achieve parallel imaging characterization of the local specificity and global morphology of the sample

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