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Bacterium preservation culture medium and preparation method thereof

A culture medium and bacteria technology, applied in the direction of bacteria, preserved microorganisms, etc., can solve the problems of long-term preservation of unfavorable strains, poor effect of strain restriction, and reduced activity of strains, so as to achieve long-term survival of bacteria, preservation of bacteria, Well-hydrated effect

Pending Publication Date: 2021-11-23
贵州安康医学检验中心有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, the amount of agar used in the bacterial preservation medium is large, and the restriction effect on the strains is poor. After preservation, the activity of the strains decreases, which is not conducive to the long-term preservation of the strains.

Method used

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  • Bacterium preservation culture medium and preparation method thereof
  • Bacterium preservation culture medium and preparation method thereof
  • Bacterium preservation culture medium and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A bacterial preservation medium, comprising the following components:

[0022] Based on 1000ml, it contains: beef extract 8g, peptone 2g, bile salt 0.2g, aluminum potassium sulfate 0.2g, malt extract powder 2g, yeast extract powder 3g, sodium chloride 0.3g, agar 5g, soluble iron salt 0.2g, Calcium Supplement 0.01g, D-Cycloserine 0.02g, Trichosanthone 0.001g, Vitamin K10.01g, Complexin Phosphopeptide 0.1g, Glucose 3g, Lanthanum Nitrate 1g, Erythritol 3g, Glycerin 18ml, Warfarin Sodium Tablet 0.1 mg, isoniazid 8 mg, sterile defibrinated sheep blood 75 ml; the soluble iron salt is ferric ammonium citrate; the calcium supplement is calcium lactate.

[0023] The bacterial preservation culture medium described in the present embodiment, the preparation method is as follows:

[0024] (1) According to the ratio of raw materials, take beef extract, peptone, bile salt, aluminum potassium sulfate, malt extract powder, yeast extract powder, sodium chloride, agar, soluble iron salt...

Embodiment 2

[0028] A bacterial preservation medium, comprising the following components:

[0029] Based on 1000ml: beef extract 10g, peptone 5g, bile salt 0.3g, aluminum potassium sulfate 0.25g, malt extract powder 4g, yeast extract powder 5g, sodium chloride 0.5g, agar 8g, ferric acid 0.3g, Calcium Lactate 0.03g, D-Cycloserine 0.03g, Trichosanone 0.003g, Vitamin K10.01g, Complexin Phosphopeptide 0.1g, Glucose 5g, Lanthanum Nitrate 2g, Erythritol 4g, Glycerin 20ml, Warfarin Sodium Tablets 0.1mg, isoniazid 10mg, sterile defibrinated sheep blood 80ml, distilled water added to 1000ml.

[0030] The bacterial preservation culture medium described in the present embodiment, the preparation method is as follows:

[0031] (1) According to the ratio of raw materials, take beef extract, peptone, bile salt, aluminum potassium sulfate, malt extract powder, yeast extract powder, sodium chloride, agar, soluble iron salt, calcium supplement, D-cycloserine, and Maozi Grass ketone, vitamin K1, complexin...

Embodiment 3

[0035] A bacterial preservation medium, comprising the following components:

[0036] Based on 1000ml, it contains: 13g of beef extract, 6g of peptone, 0.5g of bile salt, 0.3g of potassium aluminum sulfate, 5g of malt extract powder, 7g of yeast extract powder, 0.6g of sodium chloride, 10g of agar, 0.5g of soluble iron salt, Calcium Supplement 0.05g, D-Cycloserine 0.05g, Trichosanone 0.004g, Vitamin K10.02g, Complexin Phosphopeptide 0.3g, Glucose 5g, Lanthanum Nitrate 2.5g, Erythritol 5g, Glycerin 23ml, Warfarin 0.2 mg of sodium tablets, 12 mg of isoniazid, 85 ml of sterile defibrinated sheep blood; the soluble iron salt is ferric ammonium citrate; the calcium supplement is calcium lactate.

[0037] The bacterial preservation culture medium described in the present embodiment, the preparation method is as follows:

[0038] (1) According to the ratio of raw materials, take beef extract, peptone, bile salt, aluminum potassium sulfate, malt extract powder, yeast extract powder, ...

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Abstract

The invention relates to the technical field of strain preservation, in particular to a bacterium preservation culture medium and a preparation method thereof. The culture medium comprises the following components: based on 1000ml of the culture medium, 8 to 13 g of beef extract, 2 to 6 g of peptone, 0.2 to 0.5 g of cholate, 0.2 to 0.3 g of aluminum potassium sulfate, 2 to 5 g of malt extract powder, 3 to 7 g of yeast extract powder, 0.3 to 0.6 g of sodium chloride, 5 to 10 g of agar, 0.2 to 0.5 g of soluble ferric salt, 0.01 to 0.05 g of a calcium supplement, 0.02 to 0.05 g of D-cycloserine, 0.001 to 0.004 g of argentein, 0.01 to 0.02 g of vitamin K1, 0.1 to 0.3 g of casein phosphopeptides, 3 to 5 g of glucose, 1 to 2.5 g of lanthanum nitrate, 3 to 5 g of erythritol, 18 to 23 ml of glycerin, 0.1 to 0.2 mg of warfarin sodium tablets, 8 to 12 mg of isoniazide, and 75 to 85 ml of sterile defiberized sheep blood. The formula of the culture medium is rich and comprehensive in nutrient substances, the survival of the strain can be ensured, and the culture medium can still ensure the effectiveness of the strain even if the preservation environment temperature is greatly changed.

Description

technical field [0001] The invention relates to the technical field of bacterial strain preservation, in particular to a bacterial preservation medium and a preparation method thereof. Background technique [0002] Bacteria are one of the main biological resources and are frequently used in medical experiments. After an excellent strain is bred, it must keep its excellent traits unchanged or slow down as little as possible, so as not to reduce the performance of the bacteria and can be used in production for a long time. There are many methods of strain preservation. Cryopreservation is an effective way to solve germplasm degradation and prevent natural accumulation mutations, but traditional cryopreservation requires expensive programmed cooling equipment and cumbersome steps. Complete vitrification is a new method of cryopreservation, that is, under high concentration of protective agent, the cells and the protective agent will enter the vitrified state during rapid cooli...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/04
CPCC12N1/20C12N1/04
Inventor 詹琳张梦云耿见忠黄山郝家明陈兰杨璐杨红周杰张彪吴佳连
Owner 贵州安康医学检验中心有限公司
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