Grate electrode modification method, grate-controlled graphene transistor biosensor and miRNA concentration detection method

Abstract

The invention provides a gate electrode modification method, a gate-controlled graphene transistor biosensor and a miRNA concentration detection method. The gate electrode modification method comprises the following steps: adding chitosan into a streptavidin solution to obtain a first mixture; dropwise adding the first mixture onto a gate electrode, and then dropwise adding an HP-2 nucleic acid solution for cultivation to obtain an HP-2 modified gate electrode; mixing a target object miRNA141 and ST/HP-1 hybrid nucleic acid, and then adding T7 exonuclease for treatment to obtain a second mixture; and dropwise adding the second mixture to an HP-2 modified gate electrode. According to the gate electrode modification method, the second mixture is dropped on the HP-2 modified gate electrode, a single chain in the solution is captured by the HP-2 modified electrode, and the second mixture modified electrode is formed. In the enzyme digestion circulation, the T7 exonuclease (T7exo) can be used for replacing and amplifying the miRNA-141.

Description

technical field [0001] The invention relates to the technical fields of chemical detection and biosensing, in particular to a gate electrode modification method, a gate-controlled graphene transistor biosensor and a miRNA-141 concentration detection method. Background technique [0002] Highly stable circulating microRNAs (miRNAs) have emerged as the most promising and minimally invasive cancer biomarkers and are widely present in animals, plants, and microorganisms. It has the following two characteristics: 1) short non-coding endogenous RNA molecules (usually 19-22 nucleotides); 2) can regulate the expression of specific genes at the post-transcriptional level (mainly through complementary binding inhibition or degradation Messenger RNA (mRNA) acts as a regulator at the post-transcriptional level). And their abnormal expression is often observed in diseases such as Parkinson's disease, major depression, stroke and various cancers. A series of miRNAs, including miRNA-141,...

AI Technical Summary

Problems solved by technology

Since the transistor itself has an amplification function, it can be detected through the transistor. At present, the solution gate transistor is mainly detected through the interaction between the analyte and the channel, so the channel modification is more complicated, which makes the stability of the device poor.

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