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Insect thin film virus feeding method and application thereof

A film-based technology for feeding poison and insects, applied to measuring devices, instruments, scientific instruments, etc., can solve the problems of complex process, inconvenient, and inability to guarantee the rate of aphid transmission, and achieve the effect of simple and convenient operation and improved efficiency

Pending Publication Date: 2021-11-02
INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The process of this method is complicated and inconvenient, and it is necessary to judge whether the aphids have started to pierce and suck. There are certain subjective judgment factors that affect the operation process, and the transmission rate of the aphids cannot be guaranteed.

Method used

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  • Insect thin film virus feeding method and application thereof
  • Insect thin film virus feeding method and application thereof
  • Insect thin film virus feeding method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Example 1 Detection of transmission efficiency of barley yellow dwarf virus (China Taiyuan diseased strain) by different geographic populations of Aphid aphid

[0077] The wheat cultivar Beijing 837 was used as the test plant to test the transmission efficiency of the Chinese Taiyuan BYDV-PAV strain from 14 geographical populations of Aphid aphid in China. The test results are shown in Table 1.

[0078] Table 1 Transmission efficiency of different geographical populations to barley yellow dwarf disease (the diseased strain in Taiyuan, China)

[0079]

[0080]

[0081] Note: a Different letters in the same column indicate significant differences at the 0.05 level (df=13, MS=8.219, F=23.5, Pc The number of non-poisonous aphids.

[0082] It can be seen from Table 1 that there are certain differences in the transmission efficiency of BYDV-PAV Chinese Taiyuan strains transmitted by different geographical populations of Aphid aphid in China. % to 56.18%. The transmis...

Embodiment 2

[0083] Example 2 Detection of transmission efficiency of barley yellow dwarf virus (Belgium Leuven strain) by different geographic populations of Aphid aphid

[0084] The wheat cultivar Beijing 837 was used as the test plant to test the transmission efficiency of 14 geographical populations of Aphid aphid in China to the BYDV-PAV strain in Leuven, Belgium. The test results are shown in Table 2.

[0085] Table 2 Transmission efficiency of different geographical populations to barley yellow dwarf disease (Belgium Leuven strain)

[0086]

[0087]

[0088] Note: a Different letters in the same column indicate significant differences at the 0.05 level (df=13, MS=8.219, F=23.5, Pc The number of non-poisonous aphids.

[0089] It can be seen from Table 2 that the Chinese tuber aphid can transmit BYDV-PAV Belgian strains, indicating that this method can successfully transmit virus strains from different geographic populations to cross-regional virus strains. The average transmi...

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Abstract

The invention belongs to the technical field of wheat aphid physiology and phytopathology, and particularly relates to a piercing-sucking type insect thin film virus feeding method and application thereof. The method comprises the following steps: (1) uniformly thinning a plastic film, and covering the upper end of a container with openings at the two ends with the plastic film; (2) dropwise adding a virus feeding solution on the thin film; (3) uniformly thinning the plastic film, and covering the virus feeding solution with the plastic film; (4) inclining the opening at the lower end of the container upwards, and putting the insects into the container through the opening at the lower end; and (5) putting the container filled with the insects onto absorbent paper with the lower end opening facing downwards, and putting the container onto a plane for virus feeding. The method is simple and convenient to operate, effectively improves the efficiency of sucking food or virus solution by the insects, and provides convenience for further research on the virus transmission efficiency between the insects and plants.

Description

technical field [0001] The invention belongs to the technical field of wheat aphid physiology and plant pathology, and in particular relates to a method and application of piercing-sucking insect film feeding poison. Background technique [0002] Barley Yellow Dwarf Viruses (BYDVs) can infect more than 150 kinds of cereal crops and grass weeds such as wheat, barley, rice, and oats. In my country, it can infect barley, wheat, rye, triticale, oats, wild oats, sweet corn, brome, golden foxtail, sagegrass, big ling grass, small teff, tufted wheat, silver scale grass and Golden foxtail, etc. Among them, spontaneous wheat seedlings, sansevieria, wild oats, golden foxtail, and small teff are the summer hosts of BYDVs. The yellow dwarf disease of wheat crops caused by it is one of the most serious viral diseases of cereal crops. The common symptoms after the plant is infected are dwarfing, yellowing, reddening and curling of leaves. The main symptom on wheat is leaf chlorosis, whi...

Claims

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Application Information

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IPC IPC(8): A01K67/033G01N33/535G01N33/543
CPCA01K67/033G01N33/54306G01N33/535
Inventor 于文娟吴晗霖张姝杨芳彭云良姬红丽
Owner INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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