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SNP molecular marker located on pig chromosome 14 and related to dead fetus number and healthy birth rate of sow and application of SNP molecular marker

A technology of molecular markers and chromosomes, which is applied in the direction of recombinant DNA technology, biochemical equipment and methods, measurement/testing of microorganisms, etc., can solve problems such as low heritability, little effect of genetic improvement, and susceptibility to environmental factors, and achieve improvement Breeding process, increasing core competitiveness, and improving the effect of core competitiveness

Active Publication Date: 2021-10-29
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

However, the number of stillbirths and the rate of healthy babies are traits of low heritability and are easily affected by environmental factors. Therefore, the genetic improvement of the number of stillbirths and the rate of healthy babies through traditional breeding has little effect

Method used

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  • SNP molecular marker located on pig chromosome 14 and related to dead fetus number and healthy birth rate of sow and application of SNP molecular marker
  • SNP molecular marker located on pig chromosome 14 and related to dead fetus number and healthy birth rate of sow and application of SNP molecular marker
  • SNP molecular marker located on pig chromosome 14 and related to dead fetus number and healthy birth rate of sow and application of SNP molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The mensuration of embodiment 1 stillborn number and healthy baby rate

[0030] The experimental pig group used in the present invention is the purebred S22 Duroc pig of the Breeding Pig Branch of Guangdong Wen's Food Group Co., Ltd., which is the core group of the Breeding Pig Branch, and the group pedigree records are detailed.

[0031] In this experiment, a total of 536 pregnant sows of S22 line Duroc were selected in this resource group, and the pigs were fed and watered ad libitum under a unified feeding standard. On the day of farrowing, the total number of litters, number of stillbirths, and number of healthy piglets were recorded. Calculation formula of healthy litter rate: healthy litter rate (%) = healthy litter number / total litter size*100%

Embodiment 2

[0033] (1) The extraction method of DNA from the ear tissue of S22 Duroc sows was extracted from the whole genome DNA according to the marked phenol-chloroform method. Using Nanodrop-ND1000 spectrophotometer to detect the quality and concentration of the DNA of the purebred plus line Duroc population. The ratio of A260 / 280 is 1.8-2.0, and the ratio of A260 / 230 is 1.7-1.9. Finally, the qualified DNA samples were uniformly diluted to 50 ng / μl.

[0034] (2) Detection of 50K SNP genotype in the whole pig genome: GeneSeek Genomic Profiler Porcine 50KSNP typing platform, using Illumina Infinium's instructions and standard procedures for chip hybridization and result scanning. Finally, the genotype data were read by GenomeStudio software. The quality control of the obtained genotype data was carried out with PLINK v1.07, and the detection rate of elimination was -6SNP markers, individuals with a detection rate <90% and family Mendelian error rates higher than 0.1 were excluded, and...

Embodiment 3

[0040] Example 3 Detection of SNP markers

[0041] (1) The target fragment containing the SNP loci significantly related to the number of stillbirths and healthy litter rate of S22 Duroc sows is a 1499bp nucleotide sequence in chromosome 14, and the upstream and downstream primers for sequence amplification are upstream primers primer-F and downstream primer primer-R, its nucleotide sequence is as follows:

[0042] Upstream primer primer-F: 5'-GTTACTTGGGTGACATTG-3';

[0043] Downstream primer primer-R: 5'-CTAAAGGGTTAGTAGTGGT-3'.

[0044] (2) PCR amplification system and condition setting

[0045] Configure a 10 μL system, including 1 μL DNA sample, 0.3 μL upstream primer, 0.3 μL downstream primer, 5 μL PCR mix, ddH 2 O 3.4 μL, PCR conditions were pre-denaturation at 95°C for 5 min, denaturation at 95°C for 30 s, annealing at 64°C for 30 s, extension at 72°C for 1.5 min, a total of 35 cycles, and a final extension at 72°C for 5 min.

[0046] (3) DNA sequence sequencing iden...

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Abstract

The invention provides an SNP molecular marker located on a pig chromosome 14 and related to the dead fetus number and the healthy birth rate of a sow and application of the SNP molecular marker. The locus of the SNP molecular marker corresponds to T-exceeding-C mutation at the 109497913bp position on the 14 chromosome of the version 11.1 of an international pig reference genome. The molecular marker is obtained through whole genome correlation analysis, and the characters of the dead fetus number and the healthy birth rate of the sow can be remarkably influenced. The invention further provides a primer pair for detecting the molecular marker. An efficient and accurate molecular marker assisted breeding technology can be established through the molecular marker and the primer pair and applied to character genetic improvement of the dead fetus number and the healthy birth rate of the sow, and therefore the dead fetus number of the sow is decreased, the healthy birth rate is increased, and then the reproductive performance of the reproductive sow is improved.

Description

technical field [0001] The invention relates to a SNP molecular marker which is located on the No. 14 chromosome of pigs and is related to the number of stillbirths and the healthy litter rate of sows and its application. Background technique [0002] The reproductive performance of sows directly affects the economic benefits of breeding enterprises, and the number of stillbirths and healthy litter rate, as important economic traits of sows, is an important indicator to measure the reproductive performance of sows. As we all know, the number of stillbirths is negatively correlated with the healthy birth rate, the lower the number of stillbirths, the higher the healthy birth rate. Therefore, reducing the number of stillborn sows and improving the healthy litter rate of sows has always been the main goal of breeders. However, the number of stillbirths and the rate of healthy babies are traits of low heritability and are easily affected by environmental factors. Therefore, the...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2600/172C12Q2531/113C12Q2535/122
Inventor 吴珍芳杨杰周身娉丁荣荣郑恩琴蔡更元全建平李紫聪顾婷徐铮
Owner SOUTH CHINA AGRI UNIV
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