Molecular markers and applications of tmem18 gene related to chicken growth traits
A technology of growth traits and molecular markers, applied in the determination/testing of microorganisms, biochemical equipment and methods, food processing, etc., can solve the problems of low egg production, slow early growth, low fecundity, etc., and promote rapid growth , good market demand, and improve the effect of the breeding process
Active Publication Date: 2022-07-12
SOUTH CHINA AGRI UNIV
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Problems solved by technology
However, there are still disadvantages such as low egg production, low fecundity, and slow early growth.
Method used
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[0030] 1. Experimental samples
[0031] 16-week-old Xinghua chickens were slaughtered for sampling, and the brain, hypothalamus, pituitary, heart, liver, spleen, lung, kidney, chest muscle, leg muscle, sebum, abdominal fat, and ovarian tissue were collected, and stored in liquid nitrogen for RNA extraction and Quantitative detection of mRNA.
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Abstract
The invention discloses a TMEM18 gene molecular marker related to chicken growth traits, the molecular marker includes at least one of the following molecular markers: the first base at the 5' end of the TMEM18 gene nucleotide sequence shown in SEQ ID NO: 1 is the first position, C>T base mutation at position 765, G>A base mutation at position 775, G>A base mutation at position 823, and T>C base at position 904 Base mutation, G>T base mutation at position 930, C>G base mutation at position 973, T>C base mutation at position 981, A>G base at position 1014 mutation. The invention also provides the application of the molecular marker in the genetic improvement of breeding chickens, the application can promote the rapid growth of Xinghua chickens, especially to accelerate the genetic progress of the breeding of Xinghua chickens' growth traits, and shorten the breeding time of Xinghua chickens, Improve economic efficiency.
Description
technical field [0001] The invention relates to the technical field of molecular marker-assisted selection and animal genetics and breeding, in particular to a TMEM18 gene molecular marker related to chicken growth traits and its application. Background technique [0002] Xinghua Chicken is a famous chicken in Guangdong Province, named after its production in Xinghua Town, Fengkai County. The characteristics of Xinghua chicken are "two thin (thin head, thin bones), three yellows (yellow mouth, yellow feet, yellow hair), three short (short neck, short feet, short body)", plump body, and the chicken's neck is short. The weight is generally about 2.3 pounds. The apricot chicken has a unique and distinctive flavor. It has thin and crispy skin, tender meat and soft bones, even fat and delicious taste, especially when cooked with white cuts. Because the apricot chicken is raised for a long time (usually 150-180 days), the cost is high, and the taste is different, so its price i...
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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124Y02P60/87
Inventor 聂庆华阿巴朗黄卫玲黎镇晖郑茗徐海平
Owner SOUTH CHINA AGRI UNIV
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