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Stem cell medicine for treating diabetes

A technology of stem cells and diabetes, applied in the field of biomedicine, can solve the problem of decreased sensitivity of insulin signaling pathway

Pending Publication Date: 2021-10-29
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In vitro, TNF can stimulate the inflammatory signaling pathway in muscle cells, which directly leads to a decrease in the sensitivity of the insulin signaling pathway

Method used

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  • Stem cell medicine for treating diabetes
  • Stem cell medicine for treating diabetes
  • Stem cell medicine for treating diabetes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0208] Example 1 Extraction, Expansion, Identification and Differentiation of Mouse Muscle Stem Cells

[0209] (1) Extraction of mouse-derived muscle stem cells

[0210] 1. Take the mouse hind leg muscle tissue, cut it into pieces to form a tissue slurry, add 10mL type II collagenase (750U / mL), put it in a constant temperature shaker, incubate at 37°C for 60min, and the shaker speed is 70rpm.

[0211] 2. After incubation and digestion, use complete medium (containing 10% FBS) to neutralize, and centrifuge to remove the supernatant.

[0212]3. Add 10 mL of mixed digestion solution of type II collagenase (100 U / mL) and dispase (1.1 U / mL) to the pelleted cell mass again, and resuspend the cell pellet.

[0213] 4. Put the cell suspension into the constant temperature shaker again to incubate and digest. The incubation condition is 37°C for 60min, and the shaker speed is 70rpm.

[0214] 5. After the incubation, add neutralizing solution to stop the digestion, centrifuge the resul...

Embodiment 2

[0225] Example 2 Mouse high-fat diet-induced insulin resistance model and transplantation of mouse muscle stem cells

[0226] (1) Establishment of mouse model

[0227] 4-week-old male C57 mice were randomly selected and divided into groups, and fed with a normal diet containing 10% Kal fat and a high-fat diet containing 60% Kal fat, respectively. The body weight of the mice was recorded every day to form a body weight change curve. After 2-3 months of modeling, mice with a body weight of about 40 grams were selected for the experiment. High-fat diet-fed mice develop insulin resistance due to obesity and can be used as a model of type 2 diabetes.

[0228] (2) In vivo transplantation of mouse-derived muscle stem cells

[0229] 1. After the model is established, the mice are divided into experimental groups, which are divided into control group (normal diet group), model group (high-fat diet group) and treatment group (high-fat diet mouse group injected with mouse muscle stem ...

Embodiment 3

[0233] Example 3 Regulatory Effect of Mouse-derived Muscle Stem Cells on Glucose Metabolism

[0234] (1) Detection of Glucose Tolerance (GTT)

[0235] 1. Fifteen hours before the test, the mice were given fasting and water treatment.

[0236] 2. Detect the fasting blood glucose of the mice with a Bino blood glucose meter and blood glucose test strips, and record it as G0.

[0237] 3. Administer glucose to the mouse intraperitoneally, and the injection amount is 1g / g mouse. Immediately after the injection, the time at this moment was recorded as T0.

[0238] The mouse blood glucose was measured again after 15min (T15), 30min (T30), 45min (T45), 60min (T60), and 120min (T120), and recorded as G15, G30, G45, G60, G120 respectively.

[0239] (2) Detection of insulin resistance (ITT)

[0240] 1. Use the Bino blood glucose meter and blood glucose test strips to detect the fasting blood glucose of the mice, and record it as G0.

[0241] 2. Give the mice intraperitoneal injection...

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Abstract

The invention provides an application of muscle stem cells to preventing, relieving and treating metabolic disorder diseases. Researches find that the muscle stem cells can significantly improve the glycometabolism ability of the body, effectively control the blood glucose level, reduce white adipose tissue in the body, relieve the swelling and increase of adipose cells, improve the steady state of the white adipose tissue, reduce the inflammatory environment of the body, reduce the lesion degree of fatty liver and the like. In addition, the invention also provides a prevention and treatment method of muscle stem cells for increasing muscle volume and strength.

Description

technical field [0001] The invention relates to the application of muscle stem cells in the preparation of drugs for treating diabetes, and belongs to the technical field of biomedicine. Background technique [0002] With the development of social economy, changes in people's lifestyles (increased energy intake and reduced exercise, etc.) and population aging, the incidence of type 2 diabetes is increasing year by year globally, especially in developing countries. Fast (expected to increase by 170% from now to 2025), showing a strong epidemic trend. Diabetes has become the third non-communicable disease that threatens the health and life of the population after cardiovascular disease and tumors. Obesity causes increased adipose tissue or fat infiltration in tissues such as liver and muscle, and the tissues of the body change from a state of inhibiting inflammation to a state of promoting inflammation, thereby destroying tissue homeostasis. The occurrence of tissue inflamma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/34A61K45/06A61P3/10A61P1/16A61P3/06A61P5/50A61P3/00
CPCA61K35/34A61K45/06A61P3/10A61P1/16A61P3/06A61P5/50A61P3/00A61K2300/00A61P31/00A61P21/00C12N5/0659C12N2501/25C12N2501/24C12N2501/2301C12N2501/2313C12N2500/25C12N2533/54C12N2533/90A61K35/28A61P3/04
Inventor 不公告发明人
Owner SUZHOU UNIV
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