Intracellular and external electrophysiological recording system and method for automatic electroporation regulation and screening

Abstract

The invention provides an intracellular and external electrophysiological recording system and method for automatic electroporation regulation and screening. The system comprises a microelectrode array sensor, an intracellular and external electrical signal conditioning module, an electroporation control module, a data acquisition card, an upper computer and a power supply module. By means of the system, the functions of cell electroporation and myocardial cell intracellular and extracellular electric signal recording can be achieved on one instrument, and the channels can be controlled to generate pulse signals with different frequencies, amplitudes and pulse widths one by one and apply the pulse signals to cells. Therefore, comparison of electroporation results under different conditions in one experiment can be realized, change of cell electrophysiological phenomena can be detected, and cell electric signal recording with high temporal-spatial resolution is realized. Meanwhile, efficient and automatic analysis can be realized through the upper computer, the optimal condition of electroporation is screened out by traversing different electroporation conditions and combining data analysis, and cell electroporation is performed under the optimal condition, so that intracellular electric signals of myocardial cells can be recorded in a high-quality manner.

Description

technical field [0001] The invention belongs to the field of biomedical engineering, and relates to a system and method for intracellular and extracellular electrophysiological recording for automatic electroporation regulation and screening. Background technique [0002] Since intracellular electrical signals can provide action potential signals with high signal-to-noise ratio, in order to obtain high-quality action potential signals, electroporation technology based on planar electrodes is usually applied to the electrodes to rupture the cell membrane surface, so that the electrodes can record Intracellular electrical signals. At present, the signal generator is usually used as the electroporation device, and its channel number is limited, so it cannot take advantage of the electric field generated by using two or several microelectrodes in the microelectrode array. In addition, all channels of the existing instrument perform electroporation at the same time. There will b...

AI Technical Summary

Problems solved by technology

Currently, a signal generator is usually used as an electroporation device, and its number of channels is limited, so it cannot take advantage of the electric field generated by using two or several microelectrodes in a microelectrode array. In addition, all channels of existing instruments perform electroporation at the same time. There will be crosstalk between channels, and the efficiency of electroporation is low

In addition, in the experiment of detecting intracellular electrical signals, the experimenters need to test different electroporation conditions, the workload is heavy, and the operation is complicated, which has become an obstacle for in-depth study of the electrophysiological phenomena of cardiomyocytes.

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