Microfluidic chip, method, kit and system for detecting protein antigen

Abstract

The invention relates to a micro-fluidic chip, a method, a kit and a system for detecting a protein antigen. The method for detecting the protein antigen comprises the following steps: mixing a to-be-detected sample with a reaction reagent, preparing the mixture of the to-be-detected sample and the reaction reagent into a plurality of liquid drops by adopting a microfluidic technology, wherein the reaction reagent comprises a marker labelled antibody and an electrolyte providing agent, the marker labelled antibody can be specifically combined with the protein antigen in a to-be-detected sample to form an antigen-antibody compound, a plurality of antigen-antibody compounds are wrapped in the liquid drop, and the plurality of antigen-antibody compounds can be subjected to agglutination reaction under the action of the electrolyte providing agent; and detecting the intensity of a signal corresponding to the agglutination reaction in each liquid drop formed by the to-be-detected sample and the reaction reagent after the agglutination reaction, and analyzing to determine the amount of the protein antigen in the to-be-detected sample. The detection method is simple and convenient, does not need multi-step sample adding and cleaning, and is small in reagent dosage and good in reproducibility.

Description

technical field [0001] The invention relates to the technical field of immunodetection, in particular to a microfluidic chip, method, kit and system for detecting protein antigens. Background technique [0002] Digital microfluidic analysis technology based on microdroplet technology is a rapid analysis technology developed in recent years. Its largest application scenario is applied to digital PCR (digital PCR, dPCR). Its main advantage is that microfluidic droplet technology generates droplets The speed can be at the millisecond level, and thousands of droplets with good monodispersity can be generated in a few seconds, and at the same time, the size of the droplets can be precisely controlled, reaching the nanoliter or even femtoliter level. Droplet microfluidics enables independent control of the mixing, transport, and analysis of individual droplets, rapidly producing a large number of uniform droplets in a single experiment, enabling a large number of parallel reaction...

AI Technical Summary

Problems solved by technology

[0004] Therefore, in recent years, researchers have tried to apply droplet microfluidic chip technology to immune analysis, but protein molecules are different from nucleic acid DNA or RNA detection. The detection of protein molecules requires the use of biological signal markers for binding reactions to detect the presence or absence of signals. To determine the presence or absence of substances, the sandwich method is mainly used to detect antigens or antibodies. The detection process includes many times of adding samples and cleaning, and the operation is cumbersome.

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