Characteristic polypeptide for identifying deer-horn glue of Cervus nippon or Cervus elaphus, and application of characteristic polypeptide
A technology of sika deer and antler glue, applied in the fields of peptides, instruments, analytical materials, etc., can solve the problems of less research data, less research materials, and lack of protein databases on collagen and keratin, and achieve excellent specificity and stability. The effect of strong specificity and good application prospect
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[0030] 1, measure conditions
[0031] 1.1 Nanasheng liquid phase - high resolution mass spectrometry conditions
[0032] Na Raise Conditions: Separation of thermo Acclaim Pepmap C18 (100 μm × 3.5 cm, 5 μm), Isolation of thermo AccLaim Pepmap C18 (75μm × 15cm, 3 μm), flow rate 300nl / min, mobile phase A is 2 The 0.1% aqueous formation of% acetonitrile, the mobile phase B is a 5.1% aqueous formic acid containing 98% acetonitrile, and gradient elution (0 ~ 1 min, 1% B → 6% B; 1 to 96 min, 6% B → 22% B) 96 ~ 113min, 22% B → 30% B; 113 to 117 min, 30% B → 95% B; 117 to 120 min, 95% B) were 1 μm. High scoretric spectrometry: Ion source is NanoSpray Flex njun. Analysis in positive ion mode, the spray voltage is 1800 V, the ion-transport capillary temperature is 275 ° C, and the S-Lens transport efficiency is set to 60%. The primary mass spectrum is used as a mass analyzer with a resolution of 60000. The acquisition range is from 350 to 1550 (m / z); the secondary mass spectrum is used a...
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