Method for accurately and visually constructing stem cell gel in situ and matched diagnosis and treatment system
A diagnosis and treatment system and stem cell technology, applied in the field of precise visualization of in situ construction of stem cell gels, can solve problems such as difficulty in cell survival and low tissue repair efficiency, and achieve the effect of improving gel forming efficiency and realizing regeneration and repair technology.
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Embodiment 1
[0056] Basic as attached figure 1 to attach Figure 7 As shown, a supporting diagnosis and treatment system for accurately and visually constructing stem cell gel in situ includes a micro-injection pump 1. The micro-injection pump 1 includes a display screen 11, a setting button 12, a foot switch 14, and a drive motor 13. The micro-injection pump 1 is an existing device, and a syringe 15 driven by a drive motor 13 is installed on the microinjection pump 1 .
[0057] In this embodiment, an outer trocar needle 21 is also included. The outer trocar needle 21 includes a needle handle 211. The needle handle 211 is provided with an interface I214 and an interface II215. The needle handle 211 is fixed with a needle body 212 communicating with the interface I214 and the interface II215. .
[0058] In this embodiment, when the outer trocar needle 21 is combined with the existing puncture needle 26, as attached Figure 4 As shown, the outer trocar 21 is detachably connected to the pu...
Embodiment 2
[0082] The difference between the second embodiment and the first embodiment is that the attached Figure 8 And attached Figure 9 As shown, the difference is between S4 and S5.
[0083] S4: Use a set of diagnosis and treatment trocars 2 to puncture into one side of the tissue, take out the puncture needle 26, connect the optical fiber base 24 to the trocar interface II215, and connect the plug 25 to the interface I214. Use another group of diagnosis and treatment trocars 2 to puncture to the inside of the other side of the tissue, take out the puncture needle 26, connect the infusion connector 22 on the outer needle interface II215, and connect the plug 25 to the interface I214.
[0084] S5: Turn on the white light source device 35, and the white light source device 35 irradiates white light through the beam splitter 31, the optical cable 32, the optical fiber base 24 connected to the diagnosis and treatment trocar 2, and the head of the optical cable rod 37, and then the co...
Embodiment 3
[0087] This embodiment provides a gel with strong adhesion, and the preparation method includes the following steps:
[0088] S1: (PRP / GelMA solution) gel solution preparation:
[0089] (1) Configure 0.5% (w / v) photoinitiator LAP solution
[0090] S11: Weigh 0.05g of LAP on an electronic balance and put it into a brown bottle
[0091] S12: Take 10ml of PBS solution and add it into a brown bottle containing 0.05g of photoinitiator for later use
[0092] S13: Heat and dissolve in a water bath at 40-50°C for 15 minutes, shaking several times during the period
[0093] (2) The configuration concentration is 5% (w / v) GelMA solution
[0094] S11: Prepare two clean centrifuge tubes, marked as A and B respectively, and wrap them in tin foil to avoid light
[0095] S12: Weigh 0.25g of GelMA gelatin on an electronic balance and put them into centrifuge tubes A and B
[0096] S13: Use a pipette gun to take the above 2.5ml prepared 0.5% (w / v) photoinitiator solution and 2.5ml PBS sol...
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