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Bacillus coagulans with hypoglycemic effect and application thereof

A Bacillus coagulans, hypoglycemic technology, applied in the direction of application, bacteria, food ingredient functions, etc., can solve the problems such as the application of Bacillus coagulans, to improve glucose intolerance, reduce fasting blood sugar value, good drop The effect of lipolysis

Active Publication Date: 2021-09-28
JILIN MINGZHIYUAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] As of now, there is no application of Bacillus coagulans in the prevention and / or treatment of diabetes

Method used

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  • Bacillus coagulans with hypoglycemic effect and application thereof
  • Bacillus coagulans with hypoglycemic effect and application thereof
  • Bacillus coagulans with hypoglycemic effect and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Isolation, identification and preservation of embodiment 1 bacillus coagulans bacterial strain

[0042] 1. Isolation of Bacillus coagulans strains

[0043] Sample material: sauerkraut, a traditional fermented food.

[0044] In October 2019, samples of freshly pickled Laotan sauerkraut were collected from Chengdu, Sichuan Province, put into transport medium, and quickly placed in an ice box and sent to the laboratory for strain isolation. Treat sauerkraut samples in a water bath at 80°C for 10 minutes, then grind, serially dilute, and spread evenly on LB solid medium plates, culture at 50°C for 48 hours, pick a single colony and inoculate on LB medium plates to continue culturing, and then pass through LB medium The plate was streaked repeatedly for purification, and several pure colonies were obtained. The purely cultured strains were inoculated into liquid LB medium for culture, then added with 60% glycerol, and stored in a -80°C refrigerator. One of the strains was...

Embodiment 2

[0051] Example 2 Tolerance experiment of Bacillus coagulans (Bacillus coagulans) MZY531 to artificial simulated gastrointestinal fluid

[0052] Inoculate the cryopreserved Bacillus coagulans (Bacillus coagulans) MZY531 in LB medium, culture it in a shaker at 50°C at 180rpm for 16 hours, and subculture 2 to 3 times in LB medium, take 1 mL of Bacillus coagulans (Bacillus coagulans) MZY531 mixed with 9.0 mL of artificial simulated gastric juice at pH 2.5 (LB medium containing 1% pepsin, pH = 2.5), and cultured at 50°C on a shaker at 180 rpm, at 0h, 0.5h, 1h and Samples were taken at 2 hours, poured and cultivated with LB agar medium, and plate colonies were counted to determine the number of viable bacteria and calculate their survival rate. The survival rate refers to the ratio of the logarithmic value of the number of viable bacteria when sampling in the culture solution to the logarithmic value of the number of viable bacteria when sampling at 0h, expressed in %.

[0053] Tak...

Embodiment 3

[0059] Example 3 Evaluation of hypoglycemic effect of Bacillus coagulans (Bacillus coagulans) MZY531

[0060] 1. Preparation of bacterial suspension

[0061] Inoculate Bacillus coagulans (Bacillus coagulans) MZY531 in liquid LB medium, culture at 50°C at 180rpm for 16h, 3000r / min, and centrifuge at 4°C for 10min, discard the supernatant to obtain bacterial precipitates, use sterile saline for the sludge Prepare a bacterial suspension, adjust the number of bacteria to 1×10 according to the OD600 absorbance and plate count results 8 CFU / mL bacterial suspension.

[0062] 2. Diabetes model establishment and grouping

[0063] After the male C57BL / 6J mice were acclimatized for one week, the normal group was fed with normal diet, and the experimental groups were fed with high-fat and high-sugar diet. The mice in each group were free to eat and drink, and their body weight was monitored once a week. After 8 weeks, they were fasted for 12 hours. The mice in the experimental group we...

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Abstract

The invention discloses bacillus coagulans with a hypoglycemic effect and application thereof, and relates to the field of food microorganisms. The bacillus coagulans MZY531 is preserved in the China Center for Type Culture Collection on June 2, 2021; and the preservation number of the bacillus coagulans MZY531 is CCTCC NO: M2021662.The invention further discloses the application of the bacillus coagulans MZY531 in preparation of a hypoglycemic product. The bacillus coagulans MZY531 has better tolerance to artificial simulated gastrointestinal fluid, has a treatment effect on hyperglycemia of type II diabetes, and can obviously reduce the fasting blood-glucose value; the blood glucose level of a mouse with the type II diabetes can be well adjusted; the glucose intolerance condition of the mouse with the type II diabetes is improved; a good lipid-lowering effect is achieved; the host energy balance can be adjusted; and the effect of regulating the intestinal flora balance of the mice with the type II diabetes mellitus can be achieved.

Description

technical field [0001] The invention relates to the technical field of food microorganisms, in particular to a strain of Bacillus coagulans with hypoglycemic effect and application thereof. Background technique [0002] Diabetes mellitus is a metabolic disorder characterized by persistent hyperglycemia and has become a major public health problem. The causes of diabetes mainly include genetic factors and environmental factors. Type 2 diabetes suffers from metabolic disorders caused by insulin deficiency and insulin dysfunction, including sugar, lipid and protein, which in turn causes a series of complications leading to organ failure. Treatment with common drugs such as metformin and miglitol tablets can cause damage to the liver, kidneys and other organs of the human body, causing multiple organ failure and even death. Therefore, there is an urgent need to find a safe, non-toxic, and side-effect-free treatment to treat diabetes. [0003] Probiotics are a type of live mic...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/742A61P3/10A23L33/135A23L19/00A23C9/12A23C9/16A23L11/50C12R1/07
CPCA61K35/742A61P3/10A23L33/135A23L19/00A23C9/1203A23C9/16A23L11/50A23V2002/00A23V2200/328A23V2200/3204Y02A50/30
Inventor 崔伟东
Owner JILIN MINGZHIYUAN BIOTECH
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