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Elsholtzia extract, and preparation method and application thereof

A kind of Rosaceae extract and extract technology, which is applied in the field of whitening plant extract and its preparation, can solve the problems such as no whitening activity reports, and achieve good whitening activity effect

Pending Publication Date: 2021-09-24
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Golden eriothroxanthin has anti-allergic and anti-inflammatory activities. It has been reported in the field of cosmetics that it can effectively protect against damage caused by ultraviolet rays, has strong ultraviolet absorption, anti-inflammatory, and anti-allergic effects, but it has not been seen. Whitening activity report

Method used

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  • Elsholtzia extract, and preparation method and application thereof
  • Elsholtzia extract, and preparation method and application thereof
  • Elsholtzia extract, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The preparation of embodiment 1 Elderberry extract

[0017] (1) placing the Herba Azalea powder in 60% ethanol solution and extracting by percolation to obtain an ethanol extract, reclaiming the ethanol solvent to obtain the first extract; (2) adding a small amount of water to the first extract, Form a suspension, and adjust the pH value of the suspension between 4.5-6.0; (3) add an appropriate amount of cellulase to the suspension, and react in a water bath at 45°C for 60 minutes; (4) transfer the reaction solution into a Soxhlet extractor, and add 95% Ethanol, leaching for 90min, filtered and separated after cooling, and the filtrate was concentrated to prepare the target extract (hereinafter referred to as sample 1).

Embodiment 2

[0018] Embodiment 2 Comparison of different preparation methods of the extract of Azalea radix

[0019] Put the Herbs officinalis powder in 60% ethanol solution and extract by percolation method to obtain ethanol extract, recycle the ethanol solvent, and obtain the ethanol extraction sample (hereinafter referred to as sample 2)

[0020] Put the powder of Herbs officinalis in a 60% ethanol solution and extract by percolation to obtain an ethanol extract, recover the ethanol solvent to obtain the first extract, extract the first extract with ethyl acetate, combine the extracts, and recover to obtain acetic acid Ethyl ester sample (hereinafter referred to as sample 3)

[0021] Put the Herbs officinalis powder in a 60% ethanol solution and extract by percolation to obtain an ethanol extract, recover the ethanol solvent to obtain the first extract, extract the first extract with n-butanol, combine the extracts, and recover to obtain n-butanol. Butanol sample (hereinafter referred ...

Embodiment 3

[0029] Embodiment 3 different preparation methods tyrosinase activity comparison

[0030] As a person skilled in the art, everyone knows that the parts extracted by different extraction methods contain different compounds and have different activities. On the premise that all the chemical components are not clear, the activities of the samples prepared in Example 1 and Example 2 were compared through the tyrosine activity.

[0031] Determination of tyrosinase (TYR) activity: Measure 3mL of 50mmol / L PBS (pH=6.8), add 2.9mL of 1mmol / L levodopa solution, place it in a water bath at 32°C for 20min, add 0.1mL of TYR solution (0.01g / L) was mixed, and the growth curve of the absorbance of the mixed solution with time was measured under constant temperature conditions. It is stipulated that the absorbance value of the reaction solution at a wavelength of 475nm increases by 0.001 per minute as a TYR activity unit (U / min), and kojic acid is used as a positive control.

[0032] Calcula...

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PUM

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Abstract

The invention provides an elsholtzia extract, and a preparation method and an application thereof, and belongs to the technical field of plant extracts. The elsholtzia extract comprises 0.62-3.78 mg / g of apigenin, 2.19-4.44 mg / g of baicalein-7-methyl ether, 30-120 [mu] g / g of quercetin, 0.79-3.29 mg / g of kaempferol, 34-120 [mu] g / g of luteolin and 4.22-6.67 [mu] g / g of chrysoeriol. The elsholtzia extract is prepared by a percolation assisted enzymolysis process. The elsholtzia extract can achieve a whitening effect by inhibiting the activity of tyrosinase.

Description

technical field [0001] The invention belongs to the technical field of plant extracts, and in particular relates to a whitening plant extract and its preparation method and application. Background technique [0002] With the improvement of people's living standards and aesthetic standards, smooth and fair skin is more and more respected by women, and the proportion of whitening products in the daily chemical product market is also increasing. Due to the safe and healthy performance of natural functional substances, daily chemical products containing natural plant whitening functional ingredients have gradually become the consumer objects that people respect and the product orientation of the industry. The commonly used commercialized natural whitening extract is glabra root extract , peony root extract and tea extract etc. [0003] Tyrosinase inhibitors from natural sources are attracting more and more attention due to their safety, effectiveness, and ability to effectively...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9789A61K8/49A61Q19/02
CPCA61K8/9789A61K8/498A61Q19/02A61K2800/5922A61K2800/782A61K2800/805
Inventor 韦敏汤毅宋萍萍汤承翰颜露钱怡云王晨
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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